PIM1 抗体 (Internal Region)
Quick Overview for PIM1 抗体 (Internal Region) (ABIN6264220)
抗原
See all PIM1 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- Internal Region
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特异性
- Pim-1 Antibody detects endogenous levels of total Pim-1.
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预测反应
- Pig,Bovine,Horse,Sheep,Rabbit,Dog,Chicken
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纯化方法
- The antiserum was purified by peptide affinity chromatography using SulfoLinkTM Coupling Resin (Thermo Fisher Scientific).
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免疫原
- A synthesized peptide derived from human Pim-1, corresponding to a region within the internal amino acids.
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亚型
- IgG
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anti-Pim-1 Oncogene (PIM1) (AA 122-261) antibody
PIM1 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Pim-1 Oncogene (PIM1) (AA 1-100) antibodyPIM1 适用: 人 WB, ELISA, IF 宿主: 小鼠 Monoclonal 1C10 unconjugated
anti-Pim-1 Oncogene (PIM1) (AA 1-313) antibodyPIM1 适用: 人 ELISA, IHC (p), IF 宿主: 小鼠 Monoclonal 3F5 unconjugated
anti-Pim-1 Oncogene (PIM1) (AA 1-100) antibodyPIM1 适用: 人 WB, ELISA, IHC (p), IF 宿主: 小鼠 Monoclonal 2C9 unconjugated
anti-Pim-1 Oncogene (PIM1) (AA 129-268) antibodyPIM1 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Pim-1 Oncogene (PIM1) (AA 25-86) antibodyPIM1 适用: 人 ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Pim-1 Oncogene (PIM1) (AA 1-314) antibodyPIM1 适用: 人 ELISA, IHC, IHC (p), IF 宿主: 小鼠 Monoclonal 6A2 unconjugated
anti-Pim-1 Oncogene (PIM1) (AA 1-314) antibodyPIM1 适用: 人 ELISA, IHC, IHC (p), IF 宿主: 小鼠 Monoclonal 3F5 unconjugated
anti-Pim-1 Oncogene (PIM1) (AA 1-313) antibodyPIM1 适用: 人 ELISA, IHC (p), IF 宿主: 小鼠 Monoclonal 6A2 unconjugated
anti-Pim-1 Oncogene (PIM1) (AA 38-177) antibodyPIM1 适用: 大鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
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应用备注
- WB 1:500-1:2000, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1 mg/mL
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缓冲液
- Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- Store at -20 °C. Stable for 12 months from date of receipt.
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有效期
- 12 months
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- PIM1 (Pim-1 Oncogene (PIM1))
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别名
- PIM1
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背景
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Description: Proto-oncogene with serine/threonine kinase activity involved in cell survival and cell proliferation and thus providing a selective advantage in tumorigenesis. Exerts its oncogenic activity through: the regulation of MYC transcriptional activity, the regulation of cell cycle progression and by phosphorylation and inhibition of proapoptotic proteins (BAD, MAP3K5, FOXO3). Phosphorylation of MYC leads to an increase of MYC protein stability and thereby an increase of transcriptional activity. The stabilization of MYC exerted by PIM1 might explain partly the strong synergism between these two oncogenes in tumorigenesis. Mediates survival signaling through phosphorylation of BAD, which induces release of the anti-apoptotic protein Bcl-X(L)/BCL2L1. Phosphorylation of MAP3K5, an other proapoptotic protein, by PIM1, significantly decreases MAP3K5 kinase activity and inhibits MAP3K5-mediated phosphorylation of JNK and JNK/p38MAPK subsequently reducing caspase-3 activation and cell apoptosis. Stimulates cell cycle progression at the G1-S and G2-M transitions by phosphorylation of CDC25A and CDC25C. Phosphorylation of CDKN1A, a regulator of cell cycle progression at G1, results in the relocation of CDKN1A to the cytoplasm and enhanced CDKN1A protein stability. Promote cell cycle progression and tumorigenesis by down-regulating expression of a regulator of cell cycle progression, CDKN1B, at both transcriptional and post-translational levels. Phosphorylation of CDKN1B,induces 14-3-3-proteins binding, nuclear export and proteasome-dependent degradation. May affect the structure or silencing of chromatin by phosphorylating HP1 gamma/CBX3. Acts also as a regulator of homing and migration of bone marrow cells involving functional interaction with the CXCL12-CXCR4 signaling axis.
Gene: PIM1
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分子量
- 50kDa
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基因ID
- 5292
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UniProt
- P11309
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途径
- Glycosaminoglycan Metabolic Process
抗原
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