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LIG4 抗体 (C-Term)

This 兔 多克隆 anti-LIG4 antibody specifically detects LIG4 in WB, IHC, ELISA, IF 和 ICC. The antibody is reactive with 人, 小鼠 和 大鼠 samples.
产品编号 ABIN6262967
发货至: 中国
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Tel +86 (0512) 65829739 传真 +86 (010) 6788 5057

Quick Overview for LIG4 抗体 (C-Term) (ABIN6262967)

抗原

See all LIG4 抗体
LIG4 (Ligase IV, DNA, ATP-Dependent (LIG4))

适用

  • 75
  • 11
  • 9
  • 3
  • 3
  • 2
  • 1
  • 1
  • 1
  • 1
人, 小鼠, 大鼠

宿主

  • 74
  • 2

克隆类型

  • 54
  • 22
多克隆

标记

  • 33
  • 4
  • 4
  • 4
  • 4
  • 4
  • 4
  • 4
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
This LIG4 antibody is un-conjugated

应用范围

  • 47
  • 24
  • 18
  • 15
  • 15
  • 13
  • 13
  • 11
  • 3
  • 2
  • 1
Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC)
  • 抗原表位

    • 15
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    C-Term

    特异性

    DNA Ligase IV Antibody detects endogenous levels of total DNA Ligase IV.

    预测反应

    Pig,Zebrafish,Bovine,Horse,Rabbit,Dog,Chicken,Xenopus

    纯化方法

    The antiserum was purified by peptide affinity chromatography using SulfoLinkTM Coupling Resin (Thermo Fisher Scientific).

    免疫原

    A synthesized peptide derived from human DNA Ligase IV, corresponding to a region within C-terminal amino acids.

    亚型

    IgG
  • 应用备注

    WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    1 mg/mL

    缓冲液

    Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    储存条件

    -20 °C

    储存方法

    Store at -20 °C. Stable for 12 months from date of receipt.

    有效期

    12 months
  • 抗原

    LIG4 (Ligase IV, DNA, ATP-Dependent (LIG4))

    别名

    LIG4

    背景

    Description: Efficiently joins single-strand breaks in a double-stranded polydeoxynucleotide in an ATP-dependent reaction. Involved in DNA non-homologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination. The LIG4-XRCC4 complex is responsible for the NHEJ ligation step, and XRCC4 enhances the joining activity of LIG4. Binding of the LIG4-XRCC4 complex to DNA ends is dependent on the assembly of the DNA-dependent protein kinase complex DNA-PK to these DNA ends.

    Gene: LIG4

    分子量

    104kDa

    基因ID

    3981

    UniProt

    P49917

    途径

    DNA Damage Repair, Production of Molecular Mediator of Immune Response
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