Ku70/80 抗体
Our Local Distributor
北京 101111
Quick Overview for Ku70/80 抗体 (ABIN6262894)
抗原
适用
宿主
克隆类型
标记
应用范围
-
-
特异性
- Ku70/80 Antibody detects endogenous levels of total Ku70/80
-
交叉反应
- 人, 小鼠, 大鼠
-
纯化方法
- The antiserum was purified by peptide affinity chromatography using SulfoLinkTM Coupling Resin (Thermo Fisher Scientific).
-
免疫原
- A synthesized peptide derived from human Ku70/80
-
亚型
- IgG
-
-
anti-Ku70/80 (AA 683-732) antibody
适用: 人 IF, IHC, ELISA, WB 宿主: 兔 Polyclonal unconjugated
-
-
应用备注
- WB 1:500-1:2000,IHC 1:50-1:200,IF 1:100-1:500
-
限制
- 仅限研究用
-
-
-
状态
- Liquid
-
浓度
- 1 mg/mL
-
缓冲液
- Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
-
储存液
- Sodium azide
-
注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
储存条件
- -20 °C
-
储存方法
- Store at -20 °C.Stable for 12 months from date of receipt
-
有效期
- 12 months
-
-
- Ku70/80
-
背景
-
Description: Single-stranded DNA-dependent ATP-dependent helicase. Has a role in chromosome translocation. The DNA helicase II complex binds preferentially to fork-like ends of double-stranded DNA in a cell cycle-dependent manner. It works in the 3'-5' direction. Binding to DNA may be mediated by XRCC6. Involved in DNA non-homologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination. The XRCC5/6 dimer acts as regulatory subunit of the DNA-dependent protein kinase complex DNA-PK by increasing the affinity of the catalytic subunit PRKDC to DNA by 100-fold. The XRCC5/6 dimer is probably involved in stabilizing broken DNA ends and bringing them together. The assembly of the DNA-PK complex to DNA ends is required for the NHEJ ligation step. Required for osteocalcin gene expression. Probably also acts as a 5'-deoxyribose-5-phosphate lyase (5'-dRP lyase), by catalyzing the beta-elimination of the 5' deoxyribose-5-phosphate at an abasic site near double-strand breaks. 5'-dRP lyase activity allows to 'clean' the termini of abasic sites, a class of nucleotide damage commonly associated with strand breaks, before such broken ends can be joined. The XRCC5/6 dimer together with APEX1 acts as a negative regulator of transcription. Plays a role in the regulation of DNA virus-mediated innate immune response by assembling into the HDP-RNP complex, a complex that serves as a platform for IRF3 phosphorylation and subsequent innate immune response activation through the cGAS-STING pathway.
Gene: XRCC6
-
分子量
- 70, 82 kDa
-
基因ID
- 2547
-
UniProt
- P12956, P13010
抗原
-
