Histone H3.3 抗体 (N-Term)
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Quick Overview for Histone H3.3 抗体 (N-Term) (ABIN6262140)
抗原
See all Histone H3.3 (H3F3A) 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- N-Term
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原理
- H3F3A Antibody
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特异性
- H3F3A Antibody detects endogenous levels of total H3F3A.
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预测反应
- Pig,Zebrafish,Bovine,Chicken,Xenopus
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纯化方法
- The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
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免疫原
- A synthesized peptide derived from human H3F3A, corresponding to a region within N-terminal amino acids.
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亚型
- IgG
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应用备注
- WB 1:1000-3000, IHC 1:50-1:200 IF/ICC 1:100-1:500
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1 mg/mL
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缓冲液
- Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- Store at -20 °C. Stable for 12 months from date of receipt.
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有效期
- 12 months
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- Histone H3.3 (H3F3A) (H3 Histone, Family 3A (H3F3A))
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别名
- H3F3A
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背景
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Description: Variant histone H3 which replaces conventional H3 in a wide range of nucleosomes in active genes. Constitutes the predominant form of histone H3 in non-dividing cells and is incorporated into chromatin independently of DNA synthesis. Deposited at sites of nucleosomal displacement throughout transcribed genes, suggesting that it represents an epigenetic imprint of transcriptionally active chromatin. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
Gene: H3F3A, H3F3B
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分子量
- 19 kDa
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基因ID
- 3020, 3021
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UniProt
- P84243
抗原
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