DNMT3L 抗体 (Internal Region)
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Quick Overview for DNMT3L 抗体 (Internal Region) (ABIN6261345)
抗原
See all DNMT3L (TRDMT1) 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- Internal Region
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特异性
- DNMT3L Antibody detects endogenous levels of total DNMT3L.
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预测反应
- Pig,Dog
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纯化方法
- The antiserum was purified by peptide affinity chromatography using SulfoLinkTM Coupling Resin (Thermo Fisher Scientific).
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免疫原
- A synthesized peptide derived from human DNMT3L, corresponding to a region within the internal amino acids.
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亚型
- IgG
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anti-DNA (Cytosine-5-)-Methyltransferase 3 Like (TRDMT1) (AA 1-386) antibody
TRDMT1 适用: 人 WB, IF, IP 宿主: 兔 Polyclonal unconjugated
anti-DNA (Cytosine-5-)-Methyltransferase 3 Like (TRDMT1) (AA 147-386) antibodyTRDMT1 适用: 人 ELISA, FACS 宿主: 小鼠 Monoclonal 2D10H11 unconjugated
anti-DNA (Cytosine-5-)-Methyltransferase 3 Like (TRDMT1) antibodyTRDMT1 适用: 人, 小鼠, 大鼠 IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-DNA (Cytosine-5-)-Methyltransferase 3 Like (TRDMT1) (AA 1-234) antibodyTRDMT1 适用: 人 WB, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-DNA (Cytosine-5-)-Methyltransferase 3 Like (TRDMT1) (AA 1-386) antibodyTRDMT1 适用: 人 WB 宿主: 小鼠 Polyclonal unconjugated
anti-DNA (Cytosine-5-)-Methyltransferase 3 Like (TRDMT1) (AA 357-388), (C-Term) antibodyTRDMT1 适用: 人 WB, IHC (p) 宿主: 兔 Polyclonal RB02219 unconjugated
anti-DNA (Cytosine-5-)-Methyltransferase 3 Like (TRDMT1) (AA 150-175) antibodyTRDMT1 适用: 人 WB, ELISA, IHC, IP 宿主: 兔 Polyclonal unconjugated
anti-DNA (Cytosine-5-)-Methyltransferase 3 Like (TRDMT1) (AA 383-417), (C-Term) antibodyTRDMT1 适用: 小鼠 WB 宿主: 兔 Polyclonal RB50995 unconjugated
anti-DNA (Cytosine-5-)-Methyltransferase 3 Like (TRDMT1) (AA 151-386) antibodyTRDMT1 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-DNA (Cytosine-5-)-Methyltransferase 3 Like (TRDMT1) (AA 258-290) antibodyTRDMT1 适用: 小鼠 WB 宿主: 兔 Polyclonal RB50994 unconjugated
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应用备注
- WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:20-1:50, IP 1:20-1:50, CHIP 1:20-1:50, ELISA(peptide) 1:20000-1:40000
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1 mg/mL
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缓冲液
- Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- Store at -20 °C. Stable for 12 months from date of receipt.
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有效期
- 12 months
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- DNMT3L (TRDMT1) (DNA (Cytosine-5-)-Methyltransferase 3 Like (TRDMT1))
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别名
- DNMT3L
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背景
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Description: Catalytically inactive regulatory factor of DNA methyltransferases that can either promote or inhibit DNA methylation depending on the context (By similarity). Essential for the function of DNMT3A and DNMT3B: activates DNMT3A and DNMT3B by binding to their catalytic domain (PubMed:17687327). Acts by accelerating the binding of DNA and S-adenosyl-L-methionine (AdoMet) to the methyltransferases and dissociates from the complex after DNA binding to the methyltransferases (PubMed:17687327). Recognizes unmethylated histone H3 lysine 4 (H3K4me0) and induces de novo DNA methylation by recruitment or activation of DNMT3 (PubMed:17687327). Plays a key role in embryonic stem cells and germ cells (By similarity). In germ cells, required for the methylation of imprinted loci together with DNMT3A (By similarity). In male germ cells, specifically required to methylate retrotransposons, preventing their mobilization (By similarity). Plays a key role in embryonic stem cells (ESCs) by acting both as an positive and negative regulator of DNA methylation (By similarity). While it promotes DNA methylation of housekeeping genes together with DNMT3A and DNMT3B, it also acts as an inhibitor of DNA methylation at the promoter of bivalent genes (By similarity). Interacts with the EZH2 component of the PRC2/EED-EZH2 complex, preventing interaction of DNMT3A and DNMT3B with the PRC2/EED-EZH2 complex, leading to maintain low methylation levels at the promoters of bivalent genes (By similarity). Promotes differentiation of ESCs into primordial germ cells by inhibiting DNA methylation at the promoter of RHOX5, thereby activating its expression (By similarity).
Gene: DNMT3L
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分子量
- 44kDa
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基因ID
- 29947
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UniProt
- Q9UJW3
抗原
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