TPX2 抗体 (C-Term)
Quick Overview for TPX2 抗体 (C-Term) (ABIN6261299)
抗原
See all TPX2 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- C-Term
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特异性
- DIL-2 Antibody detects endogenous levels of total DIL-2.
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预测反应
- Pig,Bovine,Horse,Sheep,Rabbit,Chicken,Xenopus
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纯化方法
- The antiserum was purified by peptide affinity chromatography using SulfoLinkTM Coupling Resin (Thermo Fisher Scientific).
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免疫原
- A synthesized peptide derived from human DIL-2, corresponding to a region within C-terminal amino acids.
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亚型
- IgG
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应用备注
- WB 1:1000-3000, IHC 1:200, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1 mg/mL
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缓冲液
- Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- Store at -20 °C. Stable for 12 months from date of receipt.
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有效期
- 12 months
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- TPX2 (TPX2, Microtubule-Associated, Homolog (Xenopus Laevis) (TPX2))
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别名
- TPX2
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背景
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Description: Spindle assembly factor required for normal assembly of mitotic spindles. Required for normal assembly of microtubules during apoptosis. Required for chromatin and/or kinetochore dependent microtubule nucleation. Mediates AURKA localization to spindle microtubules (PubMed:18663142, PubMed:19208764). Activates AURKA by promoting its autophosphorylation at 'Thr-288' and protects this residue against dephosphorylation (PubMed:18663142, PubMed:19208764). TPX2 is inactivated upon binding to importin-alpha (PubMed:26165940). At the onset of mitosis, GOLGA2 interacts with importin-alpha, liberating TPX2 from importin-alpha, allowing TPX2 to activates AURKA kinase and stimulates local microtubule nucleation (PubMed:26165940).
Gene: TPX2
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分子量
- 85 kDa
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基因ID
- 22974
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UniProt
- Q9ULW0
抗原
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