BUB3 抗体 (C-Term)
Quick Overview for BUB3 抗体 (C-Term) (ABIN6260331)
抗原
See all BUB3 抗体适用
宿主
克隆类型
标记
应用范围
- 
    
- 
                                            
抗原表位
 - C-Term
 - 
                                            
特异性
 - BUB3 Antibody detects endogenous levels of total BUB3.
 - 
                                            
预测反应
 - Pig,Zebrafish,Bovine,Horse,Sheep,Rabbit,Dog,Chicken,Xenopus
 - 
                                            
纯化方法
 - The antiserum was purified by peptide affinity chromatography using SulfoLinkTM Coupling Resin (Thermo Fisher Scientific).
 - 
                                            
免疫原
 - A synthesized peptide derived from human BUB3, corresponding to a region within C-terminal amino acids.
 - 
                                            
亚型
 - IgG
 
 - 
                                            
 - 
    
 - 
    
- 
                                            
应用备注
 - WB 1:500-1:2000, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000
 - 
                                            
限制
 - 仅限研究用
 
 - 
                                            
 - 
    
- 
                                            
状态
 - Liquid
 - 
                                            
浓度
 - 1 mg/mL
 - 
                                            
缓冲液
 - Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
 - 
                                            
储存液
 - Sodium azide
 - 
                                            
注意事项
 - This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
 - 
                                            
储存条件
 - -20 °C
 - 
                                            
储存方法
 - Store at -20 °C. Stable for 12 months from date of receipt.
 - 
                                            
有效期
 - 12 months
 
 - 
                                            
 - 
    
- BUB3 (Budding Uninhibited By Benzimidazoles 3 Homolog (Yeast) (BUB3))
 - 
                                            
别名
 - BUB3
 - 
                                            
背景
 - 
                        
Description: Has a dual function in spindle-assembly checkpoint signaling and in promoting the establishment of correct kinetochore-microtubule (K-MT) attachments. Promotes the formation of stable end-on bipolar attachments. Necessary for kinetochore localization of BUB1. Regulates chromosome segregation during oocyte meiosis. The BUB1/BUB3 complex plays a role in the inhibition of anaphase-promoting complex or cyclosome (APC/C) when spindle-assembly checkpoint is activated and inhibits the ubiquitin ligase activity of APC/C by phosphorylating its activator CDC20. This complex can also phosphorylate MAD1L1.
Gene: BUB3
 - 
                                            
分子量
 - 37 kDa
 - 
                                            
基因ID
 - 9184
 - 
                                            
UniProt
 - O43684
 - 
                                            
途径
 - Maintenance of Protein Location
 
抗原
 -