BRAP 抗体 (C-Term)
Quick Overview for BRAP 抗体 (C-Term) (ABIN6260302)
抗原
See all BRAP 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- C-Term
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特异性
- BRAP Antibody detects endogenous levels of total BRAP.
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预测反应
- Pig,Bovine,Horse,Sheep,Dog,Xenopus
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纯化方法
- The antiserum was purified by peptide affinity chromatography using SulfoLinkTM Coupling Resin (Thermo Fisher Scientific).
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免疫原
- A synthesized peptide derived from human BRAP, corresponding to a region within C-terminal amino acids.
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亚型
- IgG
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应用备注
- WB 1:1000-3000, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1 mg/mL
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缓冲液
- Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- Store at -20 °C. Stable for 12 months from date of receipt.
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有效期
- 12 months
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- BRAP (BRCA1 Associated Protein (BRAP))
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别名
- BRAP
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背景
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Description: Negatively regulates MAP kinase activation by limiting the formation of Raf/MEK complexes probably by inactivation of the KSR1 scaffold protein. Also acts as a Ras responsive E3 ubiquitin ligase that, on activation of Ras, is modified by auto-polyubiquitination resulting in the release of inhibition of Raf/MEK complex formation. May also act as a cytoplasmic retention protein with a role in regulating nuclear transport.
Gene: BRAP
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分子量
- 67 kDa
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基因ID
- 8315
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UniProt
- Q7Z569
抗原
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