ATG4A 抗体 (N-Term)
Quick Overview for ATG4A 抗体 (N-Term) (ABIN6260104)
抗原
See all ATG4A 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- N-Term
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特异性
- ATG4A Antibody detects endogenous levels of total ATG4A.
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预测反应
- Pig,Zebrafish,Bovine,Horse,Sheep,Rabbit,Dog,Chicken,Xenopus
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纯化方法
- The antiserum was purified by peptide affinity chromatography using SulfoLinkTM Coupling Resin (Thermo Fisher Scientific).
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免疫原
- A synthesized peptide derived from human ATG4A, corresponding to a region within N-terminal amino acids.
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亚型
- IgG
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应用备注
- WB 1:1000-3000, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1 mg/mL
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缓冲液
- Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- Store at -20 °C. Stable for 12 months from date of receipt.
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有效期
- 12 months
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- ATG4A (Autophagy related 4A Cysteine Peptidase (ATG4A))
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别名
- ATG4A
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背景
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Description: Cysteine protease required for the cytoplasm to vacuole transport (Cvt) and autophagy. Cleaves the C-terminal amino acid of ATG8 family proteins to reveal a C-terminal glycine. Exposure of the glycine at the C-terminus is essential for ATG8 proteins conjugation to phosphatidylethanolamine (PE) and insertion to membranes, which is necessary for autophagy. Preferred substrate is GABARAPL2 followed by MAP1LC3A and GABARAP. Has also an activity of delipidating enzyme for the PE-conjugated forms.
Gene: ATG4A
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分子量
- 45 kDa
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基因ID
- 115201
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UniProt
- Q8WYN0
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途径
- Autophagy
抗原
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