ARF1 抗体 (Internal Region)
Quick Overview for ARF1 抗体 (Internal Region) (ABIN6260020)
抗原
See all ARF1 抗体适用
宿主
克隆类型
标记
应用范围
-
-
抗原表位
- Internal Region
-
特异性
- ARF1 Antibody detects endogenous levels of total ARF1.
-
预测反应
- Pig,Zebrafish,Bovine,Sheep,Rabbit,Chicken,Xenopus
-
纯化方法
- The antiserum was purified by peptide affinity chromatography using SulfoLinkTM Coupling Resin (Thermo Fisher Scientific).
-
免疫原
- A synthesized peptide derived from human ARF1, corresponding to a region within the internal amino acids.
-
亚型
- IgG
-
-
-
-
应用备注
- WB 1:1000-3000, IHC 1:50-1:200, ELISA(peptide) 1:20000-1:40000
-
限制
- 仅限研究用
-
-
-
状态
- Liquid
-
浓度
- 1 mg/mL
-
缓冲液
- Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
-
储存液
- Sodium azide
-
注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
储存条件
- -20 °C
-
储存方法
- Store at -20 °C. Stable for 12 months from date of receipt.
-
有效期
- 12 months
-
-
- ARF1 (ADP-Ribosylation Factor 1 (ARF1))
-
别名
- ARF1
-
背景
-
Description: GTP-binding protein that functions as an allosteric activator of the cholera toxin catalytic subunit, an ADP-ribosyltransferase. Involved in protein trafficking among different compartments. Modulates vesicle budding and uncoating within the Golgi complex. Deactivation induces the redistribution of the entire Golgi complex to the endoplasmic reticulum, suggesting a crucial role in protein trafficking. In its GTP-bound form, its triggers the association with coat proteins with the Golgi membrane. The hydrolysis of ARF1-bound GTP, which is mediated by ARFGAPs proteins, is required for dissociation of coat proteins from Golgi membranes and vesicles. The GTP-bound form interacts with PICK1 to limit PICK1-mediated inhibition of Arp2/3 complex activity, the function is linked to AMPA receptor (AMPAR) trafficking, regulation of synaptic plasicity of excitatory synapses and spine shrinkage during long-term depression (LTD).
Gene: ARF1
-
分子量
- 20 kDa
-
基因ID
- 375
-
UniProt
- P84077
-
途径
- Transition Metal Ion Homeostasis, Inositol Metabolic Process
抗原
-