AGO2 抗体 (N-Term)
Quick Overview for AGO2 抗体 (N-Term) (ABIN6259825)
抗原
See all AGO2 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- N-Term
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特异性
- AGO2 Antibody detects endogenous levels of total AGO2.
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预测反应
- Pig,Zebrafish,Bovine,Horse,Sheep,Rabbit,Xenopus
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纯化方法
- The antiserum was purified by peptide affinity chromatography using SulfoLinkTM Coupling Resin (Thermo Fisher Scientific).
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免疫原
- A synthesized peptide derived from human AGO2, corresponding to a region within N-terminal amino acids.
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亚型
- IgG
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anti-Argonaute 2 (AGO2) (AA 1-377) antibody
AGO2 适用: 人 WB, ELISA, IF, IHC (p), RNAi 宿主: 小鼠 Monoclonal 2E12-1C9 unconjugated
anti-Argonaute 2 (AGO2) (AA 517-818) antibodyAGO2 适用: 人 WB, ELISA, IHC, ChIP 宿主: 兔 Polyclonal unconjugated
anti-Argonaute 2 (AGO2) antibodyAGO2 适用: 人, 小鼠, 大鼠 IHC 宿主: 兔 Polyclonal unconjugated
anti-Argonaute 2 (AGO2) (AA 1-100) antibodyAGO2 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Argonaute 2 (AGO2) (AA 47-115) antibodyAGO2 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-Argonaute 2 (AGO2) (AA 27-59), (N-Term) antibodyAGO2 适用: 人 WB, FACS, IHC (p) 宿主: 兔 Polyclonal RB8013 unconjugated
anti-Argonaute 2 (AGO2) (AA 1-377) antibodyAGO2 适用: 人 WB, ELISA 宿主: 小鼠 Polyclonal unconjugated
anti-Argonaute 2 (AGO2) (AA 381-480) antibodyAGO2 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 2H2 unconjugated
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应用备注
- WB 1:500-1:2000, IHC 1:50-1:200
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1 mg/mL
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缓冲液
- Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- Store at -20 °C. Stable for 12 months from date of receipt.
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有效期
- 12 months
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- AGO2 (Argonaute 2 (AGO2))
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别名
- AGO2
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背景
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Description: Required for RNA-mediated gene silencing (RNAi) by the RNA-induced silencing complex (RISC). The 'minimal RISC' appears to include AGO2 bound to a short guide RNA such as a microRNA (miRNA) or short interfering RNA (siRNA). These guide RNAs direct RISC to complementary mRNAs that are targets for RISC-mediated gene silencing. The precise mechanism of gene silencing depends on the degree of complementarity between the miRNA or siRNA and its target. Binding of RISC to a perfectly complementary mRNA generally results in silencing due to endonucleolytic cleavage of the mRNA specifically by AGO2. Binding of RISC to a partially complementary mRNA results in silencing through inhibition of translation, and this is independent of endonuclease activity. May inhibit translation initiation by binding to the 7-methylguanosine cap, thereby preventing the recruitment of the translation initiation factor eIF4-E. May also inhibit translation initiation via interaction with EIF6, which itself binds to the 60S ribosomal subunit and prevents its association with the 40S ribosomal subunit. The inhibition of translational initiation leads to the accumulation of the affected mRNA in cytoplasmic processing bodies (P-bodies), where mRNA degradation may subsequently occur. In some cases RISC-mediated translational repression is also observed for miRNAs that perfectly match the 3' untranslated region (3'-UTR). Can also up-regulate the translation of specific mRNAs under certain growth conditions. Binds to the AU element of the 3'-UTR of the TNF (TNF-alpha) mRNA and up-regulates translation under conditions of serum starvation. Also required for transcriptional gene silencing (TGS), in which short RNAs known as antigene RNAs or agRNAs direct the transcriptional repression of complementary promoter regions.
Gene: AGO2
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分子量
- 90-95 kDa
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基因ID
- 27161
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UniProt
- Q9UKV8
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途径
- Fc-epsilon Receptor Signaling Pathway, Regulatory RNA Pathways, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Ribonucleoprotein Complex Subunit Organization
抗原
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