TOP3A 抗体 (C-Term)
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- 抗原 See all TOP3A 抗体
- TOP3A (Topoisomerase (DNA) III alpha (TOP3A))
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抗原表位
- C-Term
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适用
- 人, 小鼠
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This TOP3A antibody is un-conjugated
- 应用范围
- Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC)
- 特异性
- TOP3A Antibody detects endogenous levels of total TOP3A.
- 预测反应
- Rabbit,Dog,Xenopus
- 纯化方法
- The antiserum was purified by peptide affinity chromatography using SulfoLinkTM Coupling Resin (Thermo Fisher Scientific).
- 免疫原
- A synthesized peptide derived from human TOP3A, corresponding to a region within C-terminal amino acids.
- 亚型
- IgG
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anti-Topoisomerase (DNA) III alpha (TOP3A) (Internal Region) antibody
TOP3A 适用: 人 WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Topoisomerase (DNA) III alpha (TOP3A) antibodyTOP3A 适用: 人, 小鼠 WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Topoisomerase (DNA) III alpha (TOP3A) (AA 1-100) antibodyTOP3A 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Topoisomerase (DNA) III alpha (TOP3A) (Center) antibodyTOP3A 适用: 人, Cow, 犬, 斑马鱼 WB 宿主: 兔 Polyclonal unconjugated
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- 应用备注
- WB 1:500-1:1000, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 1 mg/mL
- 缓冲液
- Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- -20 °C
- 储存方法
- Store at -20 °C. Stable for 12 months from date of receipt.
- 有效期
- 12 months
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- 抗原
- TOP3A (Topoisomerase (DNA) III alpha (TOP3A))
- 别名
- TOP3A (TOP3A 产品)
- 别名
- TOP3 antibody, DNA topoisomerase III alpha antibody, topoisomerase (DNA) III alpha antibody, Top3a antibody, TOP3A antibody
- 背景
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Description: Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone. Essential component of the RMI complex, a complex that plays an important role in the processing of homologous recombination intermediates to limit DNA crossover formation in cells. Has DNA decatenation activity.
Gene: TOP3A
- 分子量
- 115 kDa
- 基因ID
- 7156
- UniProt
- Q13472
- 途径
- DNA Damage Repair
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