POLR3A 抗体 (N-Term)
Quick Overview for POLR3A 抗体 (N-Term) (ABIN6259297)
抗原
See all POLR3A 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- N-Term
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特异性
- RPC1 Antibody detects endogenous levels of total RPC1.
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预测反应
- Pig,Zebrafish,Bovine,Horse,Rabbit,Dog,Chicken,Xenopus
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纯化方法
- The antiserum was purified by peptide affinity chromatography using SulfoLinkTM Coupling Resin (Thermo Fisher Scientific).
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免疫原
- A synthesized peptide derived from human RPC1, corresponding to a region within N-terminal amino acids.
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亚型
- IgG
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anti-Polymerase (RNA) III (DNA Directed) Polypeptide A, 155kDa (POLR3A) (N-Term) antibody
POLR3A 适用: 人 WB, ICC, IF, IP 宿主: 兔 Polyclonal unconjugated
anti-Polymerase (RNA) III (DNA Directed) Polypeptide A, 155kDa (POLR3A) (AA 392-632) antibodyPOLR3A 适用: 人 ELISA, IHC, ChIP 宿主: 兔 Polyclonal unconjugated
anti-Polymerase (RNA) III (DNA Directed) Polypeptide A, 155kDa (POLR3A) (N-Term) antibodyPOLR3A 适用: 人 ELISA, WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Polymerase (RNA) III (DNA Directed) Polypeptide A, 155kDa (POLR3A) (N-Term) antibodyPOLR3A 适用: 人, 小鼠, 猴 ELISA, WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Polymerase (RNA) III (DNA Directed) Polypeptide A, 155kDa (POLR3A) (C-Term) antibodyPOLR3A 适用: 人, 小鼠, 大鼠, 猴, 犬, 兔, 马 WB 宿主: 兔 Polyclonal unconjugated
anti-Polymerase (RNA) III (DNA Directed) Polypeptide A, 155kDa (POLR3A) (N-Term) antibodyPOLR3A 适用: 人, 小鼠, 大鼠, 猴, 犬, 兔, Cow, 小鸡, Pig, 斑马鱼 WB, IHC 宿主: 兔 Polyclonal unconjugated
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应用备注
- WB 1:500-1:1000, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1 mg/mL
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缓冲液
- Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- Store at -20 °C. Stable for 12 months from date of receipt.
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有效期
- 12 months
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- POLR3A (Polymerase (RNA) III (DNA Directed) Polypeptide A, 155kDa (POLR3A))
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别名
- POLR3A
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背景
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Description: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Largest and catalytic core component of RNA polymerase III which synthesizes small RNAs, such as 5S rRNA and tRNAs. Forms the polymerase active center together with the second largest subunit. A single-stranded DNA template strand of the promoter is positioned within the central active site cleft of Pol III. A bridging helix emanates from RPC1 and crosses the cleft near the catalytic site and is thought to promote translocation of Pol III by acting as a ratchet that moves the RNA-DNA hybrid through the active site by switching from straight to bent conformations at each step of nucleotide addition (By similarity). Plays a key role in sensing and limiting infection by intracellular bacteria and DNA viruses. Acts as nuclear and cytosolic DNA sensor involved in innate immune response. Can sense non-self dsDNA that serves as template for transcription into dsRNA. The non-self RNA polymerase III transcripts, such as Epstein-Barr virus-encoded RNAs (EBERs) induce type I interferon and NF- Kappa-B through the RIG-I pathway.
Gene: POLR3A
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分子量
- 156 kDa
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基因ID
- 11128
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UniProt
- O14802
抗原
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