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OCT4 抗体 (AA 114-163)

This anti-OCT4 antibody is a 兔 多克隆 antibody detecting OCT4 in WB, IHC 和 IHC (p). Suitable for 人, Pig, 兔 和 山羊.
产品编号 ABIN625854
发货至: 中国

Quick Overview for OCT4 抗体 (AA 114-163) (ABIN625854)

抗原

See all OCT4 (POU5F1) 抗体
OCT4 (POU5F1) (POU Class 5 Homeobox 1 (POU5F1))

适用

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人, Pig, 兔, 山羊

宿主

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克隆类型

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多克隆

标记

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This OCT4 antibody is un-conjugated

应用范围

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Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
  • 抗原表位

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    AA 114-163

    品牌

    IHC-plus™

    特异性

    Human POU5F1 / OCT4

    预测反应

    Percent identity by BLAST analysis: Human, Goat (100%) Rabbit (92%) Dog (91%) Pig (84%).

    纯化方法

    Protein A/G purified

    免疫原

    Synthetic peptide located between aa114-163 of human POU5F1 (Q01860, NP_002692). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Goat, Rabbit (100%), Marmoset (92%), Dog (91%), Bovine, Pig, Guinea pig (85%), Horse (84%), Cat (83%).

    Type of Immunogen: Synthetic peptide
  • 应用备注

    Approved: IHC, IHC-P (5 μg/mL), WB (1.25 μg/mL)

    Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

    说明

    Target Species of Antibody: Human

    限制

    仅限研究用
  • 状态

    Liquid

    溶解方式

    Distilled water

    浓度

    Lot specific

    缓冲液

    PBS, 2 % sucrose.

    注意事项

    Avoid repeat freeze-thaw cycles.

    储存条件

    4 °C,-20 °C

    储存方法

    Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
    Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
  • 抗原

    OCT4 (POU5F1) (POU Class 5 Homeobox 1 (POU5F1))

    别名

    POU5F1 / OCT4

    背景

    Name/Gene ID: POU5F1

    Synonyms: POU5F1, Oct-4, OCT3, OCT4, OTF4, POU class 5 homeobox 1, Octamer-binding protein 3, Octamer-binding protein 4, OTF-3, Oct-3, OTF3

    基因ID

    5460

    NCBI登录号

    NP_002692

    UniProt

    Q01860

    途径

    Stem Cell Maintenance
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