ATP5A1 抗体 (Internal Region)
Quick Overview for ATP5A1 抗体 (Internal Region) (ABIN6258538)
抗原
See all ATP5A1 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- Internal Region
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特异性
- ATP5A1 Antibody detects endogenous levels of total ATP5A1.
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预测反应
- Pig,Bovine,Chicken,Xenopus
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纯化方法
- The antiserum was purified by peptide affinity chromatography using SulfoLinkTM Coupling Resin (Thermo Fisher Scientific).
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免疫原
- A synthesized peptide derived from human ATP5A1, corresponding to a region within the internal amino acids.
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亚型
- IgG
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应用备注
- WB 1:500-1:1000, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1 mg/mL
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缓冲液
- Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- Store at -20 °C. Stable for 12 months from date of receipt.
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有效期
- 12 months
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- ATP5A1 (ATP Synthase, H+ Transporting, Mitochondrial F1 Complex, alpha Subunit 1, Cardiac Muscle (ATP5A1))
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别名
- ATP5A1
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背景
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Description: Mitochondrial membrane ATP synthase (F1F0 ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F1 - containing the extramembraneous catalytic core, and F0 - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F1 is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Subunits alpha and beta form the catalytic core in F1. Rotation of the central stalk against the surrounding alpha3beta3 subunits leads to hydrolysis of ATP in three separate catalytic sites on the beta subunits. Subunit alpha does not bear the catalytic high-affinity ATP-binding sites (By similarity).
Gene: ATP5A1
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分子量
- 60 kDa
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基因ID
- 498
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UniProt
- P25705
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途径
- Proton Transport, Ribonucleoside Biosynthetic Process
抗原
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