USP15 抗体 (N-Term)
Quick Overview for USP15 抗体 (N-Term) (ABIN6258347)
抗原
See all USP15 抗体适用
宿主
克隆类型
标记
应用范围
-
-
抗原表位
- N-Term
-
特异性
- USP15 Antibody detects endogenous levels of total USP15.
-
预测反应
- Pig,Bovine,Horse,Sheep,Rabbit,Dog,Chicken
-
纯化方法
- The antiserum was purified by peptide affinity chromatography using SulfoLinkTM Coupling Resin (Thermo Fisher Scientific).
-
免疫原
- A synthesized peptide derived from human USP15, corresponding to a region within N-terminal amino acids.
-
亚型
- IgG
-
-
anti-Ubiquitin Specific Peptidase 15 (USP15) (AA 1-235) antibody
USP15 适用: 人 WB, ELISA, IF 宿主: 小鼠 Monoclonal 1C10 unconjugated
anti-Ubiquitin Specific Peptidase 15 (USP15) (C-Term) antibodyKO Validated USP15 适用: 人 WB, IF, IHC (p), ICC 宿主: 兔 Polyclonal unconjugated
anti-Ubiquitin Specific Peptidase 15 (USP15) (AA 151-180), (N-Term) antibodyUSP15 适用: 人, 小鼠 WB, IF, IHC (p) 宿主: 兔 Polyclonal RB4337 unconjugated
anti-Ubiquitin Specific Peptidase 15 (USP15) (AA 852-952) antibodyUSP15 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-Ubiquitin Specific Peptidase 15 (USP15) (C-Term) antibodyUSP15 适用: 人, 小鼠, 大鼠, Cow, 犬, 马, 兔, 豚鼠 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-Ubiquitin Specific Peptidase 15 (USP15) (N-Term) antibodyUSP15 适用: 人 WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Ubiquitin Specific Peptidase 15 (USP15) (AA 1-260), (C-Term) antibodyUSP15 适用: 人 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Ubiquitin Specific Peptidase 15 (USP15) (AA 691-740) antibodyUSP15 适用: 人, 小鼠, 大鼠, Cow, 犬, 马, 兔, 非洲爪蟾, 豚鼠, 猴, Pig, Bat WB 宿主: 兔 Polyclonal unconjugated
-
-
应用备注
- WB 1:500-1:1000, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000
-
限制
- 仅限研究用
-
-
-
状态
- Liquid
-
浓度
- 1 mg/mL
-
缓冲液
- Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
-
储存液
- Sodium azide
-
注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
储存条件
- -20 °C
-
储存方法
- Store at -20 °C. Stable for 12 months from date of receipt.
-
有效期
- 12 months
-
-
- USP15 (Ubiquitin Specific Peptidase 15 (USP15))
-
别名
- USP15
-
背景
-
Description: Hydrolase that removes conjugated ubiquitin from target proteins and regulates various pathways such as the TGF-beta receptor signaling, NF-kappa-B and RNF41/NRDP1-PRKN pathways (PubMed:21947082, PubMed:22344298, PubMed:24852371, PubMed:16005295, PubMed:17318178, PubMed:19826004, PubMed:19576224). Acts as a key regulator of TGF-beta receptor signaling pathway, but the precise mechanism is still unclear: according to a report, acts by promoting deubiquitination of monoubiquitinated R-SMADs (SMAD1, SMAD2 and/or SMAD3), thereby alleviating inhibition of R-SMADs and promoting activation of TGF-beta target genes (PubMed:21947082). According to another reports, regulates the TGF-beta receptor signaling pathway by mediating deubiquitination and stabilization of TGFBR1, leading to an enhanced TGF-beta signal (PubMed:22344298). Able to mediate deubiquitination of monoubiquitinated substrates as well as 'Lys-48'-linked polyubiquitin chains, protecting them against proteasomal degradation. May also regulate gene expression and/or DNA repair through the deubiquitination of histone H2B (PubMed:24526689). Acts as an inhibitor of mitophagy by counteracting the action of parkin (PRKN): hydrolyzes cleavage of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains attached by parkin on target proteins such as MFN2, thereby reducing parkin's ability to drive mitophagy (PubMed:24852371). Acts as an associated component of COP9 signalosome complex (CSN) and regulates different pathways via this association: regulates NF-kappa-B by mediating deubiquitination of NFKBIA and deubiquitinates substrates bound to VCP (PubMed:16005295, PubMed:17318178, PubMed:19826004, PubMed:19576224). Involved in endosome organization by mediating deubiquitination of SQSTM1: ubiquitinated SQSTM1 forms a molecular bridge that restrains cognate vesicles in the perinuclear region and its deubiquitination releases target vesicles for fast transport into the cell periphery (PubMed:27368102).
Gene: USP15
-
分子量
- 115 kDa
-
基因ID
- 9958
-
UniProt
- Q9Y4E8
抗原
-
