POLD3 抗体 (C-Term)
Quick Overview for POLD3 抗体 (C-Term) (ABIN6258121)
抗原
See all POLD3 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- C-Term
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特异性
- POLD3 Antibody detects endogenous levels of total POLD3.
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预测反应
- Pig,Bovine,Horse,Sheep,Rabbit,Dog,Chicken
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纯化方法
- The antiserum was purified by peptide affinity chromatography using SulfoLinkTM Coupling Resin (Thermo Fisher Scientific).
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免疫原
- A synthesized peptide derived from human POLD3, corresponding to a region within C-terminal amino acids.
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亚型
- IgG
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anti-Polymerase (DNA-Directed), delta 3, Accessory Subunit (POLD3) (AA 197-466) antibody
POLD3 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-Polymerase (DNA-Directed), delta 3, Accessory Subunit (POLD3) antibodyPOLD3 适用: 人, 小鼠 WB, IF, IHC 宿主: 兔 Polyclonal unconjugated
anti-Polymerase (DNA-Directed), delta 3, Accessory Subunit (POLD3) antibodyPOLD3 适用: 小鼠, 大鼠 IF, IHC 宿主: 兔 Polyclonal unconjugated
anti-Polymerase (DNA-Directed), delta 3, Accessory Subunit (POLD3) (AA 357-466) antibodyPOLD3 适用: 人 WB, ELISA, IF 宿主: 小鼠 Monoclonal 3E2 unconjugated
anti-Polymerase (DNA-Directed), delta 3, Accessory Subunit (POLD3) (AA 203-275) antibodyPOLD3 适用: 人 WB, ELISA, IF, IHC 宿主: 兔 Polyclonal unconjugated
anti-Polymerase (DNA-Directed), delta 3, Accessory Subunit (POLD3) (C-Term) antibodyPOLD3 适用: 人 WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Polymerase (DNA-Directed), delta 3, Accessory Subunit (POLD3) (AA 357-466) antibodyPOLD3 适用: 人 WB, ELISA 宿主: 小鼠 Polyclonal unconjugated
anti-Polymerase (DNA-Directed), delta 3, Accessory Subunit (POLD3) (AA 1-466) antibodyPOLD3 适用: 人 WB 宿主: 小鼠 Polyclonal unconjugated
anti-Polymerase (DNA-Directed), delta 3, Accessory Subunit (POLD3) (AA 415-443), (C-Term) antibodyPOLD3 适用: 人, 小鼠 WB, EIA 宿主: 兔 Polyclonal unconjugated
anti-Polymerase (DNA-Directed), delta 3, Accessory Subunit (POLD3) (AA 416-443), (C-Term) antibodyPOLD3 适用: 人, 小鼠 WB 宿主: 兔 Polyclonal unconjugated
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应用备注
- WB 1:500-1:1000, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1 mg/mL
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缓冲液
- Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- Store at -20 °C. Stable for 12 months from date of receipt.
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有效期
- 12 months
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- POLD3 (Polymerase (DNA-Directed), delta 3, Accessory Subunit (POLD3))
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别名
- POLD3
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背景
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Description: As a component of the trimeric and tetrameric DNA polymerase delta complexes (Pol-delta3 and Pol-delta4, respectively), plays a role in high fidelity genome replication, including in lagging strand synthesis, and repair. Required for optimal Pol-delta activity. Stabilizes the Pol-delta complex and plays a major role in Pol-delta stimulation by PCNA (PubMed:10219083, PubMed:10852724, PubMed:11595739, PubMed:16510448, PubMed:24035200). Pol-delta3 and Pol-delta4 are characterized by the absence or the presence of POLD4. They exhibit differences in catalytic activity. Most notably, Pol-delta3 shows higher proofreading activity than Pol-delta4 (PubMed:19074196, PubMed:20334433). Although both Pol-delta3 and Pol-delta4 process Okazaki fragments in vitro, Pol-delta3 may also be better suited to fulfill this task, exhibiting near-absence of strand displacement activity compared to Pol-delta4 and stalling on encounter with the 5'-blocking oligonucleotides. Pol-delta3 idling process may avoid the formation of a gap, while maintaining a nick that can be readily ligated (PubMed:24035200). Along with DNA polymerase kappa, DNA polymerase delta carries out approximately half of nucleotide excision repair (NER) synthesis following UV irradiation. In this context, POLD3, along with PCNA and RFC1-replication factor C complex, is required to recruit POLD1, the catalytic subunit of the polymerase delta complex, to DNA damage sites (PubMed:20227374). Under conditions of DNA replication stress, required for the repair of broken replication forks through break-induced replication (BIR) (PubMed:24310611). Involved in the translesion synthesis (TLS) of templates carrying O6-methylguanine or abasic sites performed by Pol-delta4, independently of DNA polymerase zeta (REV3L) or eta (POLH). Facilitates abasic site bypass by DNA polymerase delta by promoting extension from the nucleotide inserted opposite the lesion (PubMed:19074196, PubMed:25628356, PubMed:27185888). Also involved in TLS, as a component of the POLZ complex. Along with POLD2, dramatically increases the efficiency and processivity of DNA synthesis of the minimal DNA polymerase zeta complex, consisting of only REV3L and REV7 (PubMed:24449906).
Gene: POLD3
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分子量
- 51 kDa
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基因ID
- 10714
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UniProt
- Q15054
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途径
- Telomere Maintenance, DNA Damage Repair, DNA Replication, Synthesis of DNA
抗原
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