NCBP2 抗体 (N-Term)
Quick Overview for NCBP2 抗体 (N-Term) (ABIN6257882)
抗原
See all NCBP2 抗体适用
宿主
克隆类型
标记
应用范围
-
-
抗原表位
- N-Term
-
特异性
- NCBP2 Antibody detects endogenous levels of total NCBP2.
-
预测反应
- Pig,Bovine,Horse,Sheep,Rabbit,Dog
-
纯化方法
- The antiserum was purified by peptide affinity chromatography using SulfoLinkTM Coupling Resin (Thermo Fisher Scientific).
-
免疫原
- A synthesized peptide derived from human NCBP2, corresponding to a region within N-terminal amino acids.
-
亚型
- IgG
-
-
anti-Nuclear Cap Binding Protein Subunit 2 (NCBP2) (AA 1-156) antibody
NCBP2 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-Nuclear Cap Binding Protein Subunit 2 (NCBP2) antibodyNCBP2 适用: 人, 小鼠, 大鼠 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Nuclear Cap Binding Protein Subunit 2 (NCBP2) (AA 1-156) antibodyNCBP2 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Nuclear Cap Binding Protein Subunit 2 (NCBP2) (N-Term) antibodyNCBP2 适用: 人, 小鼠, 大鼠, Cow, 猴 WB, IHC, IF, IC 宿主: 兔 Polyclonal unconjugated
anti-Nuclear Cap Binding Protein Subunit 2 (NCBP2) (N-Term) antibodyNCBP2 适用: 人 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Nuclear Cap Binding Protein Subunit 2 (NCBP2) (N-Term) antibodyNCBP2 适用: 人, 小鼠 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Nuclear Cap Binding Protein Subunit 2 (NCBP2) (AA 1-99) antibodyNCBP2 适用: 人 ELISA 宿主: 小鼠 Monoclonal 3A12 unconjugated
-
-
应用备注
- WB 1:500-1:1000, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000
-
限制
- 仅限研究用
-
-
-
状态
- Liquid
-
浓度
- 1 mg/mL
-
缓冲液
- Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
-
储存液
- Sodium azide
-
注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
储存条件
- -20 °C
-
储存方法
- Store at -20 °C. Stable for 12 months from date of receipt.
-
有效期
- 12 months
-
-
- NCBP2 (Nuclear Cap Binding Protein Subunit 2 (NCBP2))
-
别名
- NCBP2
-
背景
-
Description: Component of the cap-binding complex (CBC), which binds co-transcriptionally to the 5' cap of pre-mRNAs and is involved in various processes such as pre-mRNA splicing, translation regulation, nonsense-mediated mRNA decay, RNA-mediated gene silencing (RNAi) by microRNAs (miRNAs) and mRNA export. The CBC complex is involved in mRNA export from the nucleus via its interaction with ALYREF/THOC4/ALY, leading to the recruitment of the mRNA export machinery to the 5' end of mRNA and to mRNA export in a 5' to 3' direction through the nuclear pore. The CBC complex is also involved in mediating U snRNA and intronless mRNAs export from the nucleus. The CBC complex is essential for a pioneer round of mRNA translation, before steady state translation when the CBC complex is replaced by cytoplasmic cap-binding protein eIF4E. The pioneer round of mRNA translation mediated by the CBC complex plays a central role in nonsense-mediated mRNA decay (NMD), NMD only taking place in mRNAs bound to the CBC complex, but not on eIF4E-bound mRNAs. The CBC complex enhances NMD in mRNAs containing at least one exon-junction complex (EJC) via its interaction with UPF1, promoting the interaction between UPF1 and UPF2. The CBC complex is also involved in 'failsafe' NMD, which is independent of the EJC complex, while it does not participate in Staufen-mediated mRNA decay (SMD). During cell proliferation, the CBC complex is also involved in microRNAs (miRNAs) biogenesis via its interaction with SRRT/ARS2, thereby being required for miRNA-mediated RNA interference. The CBC complex also acts as a negative regulator of PARN, thereby acting as an inhibitor of mRNA deadenylation. In the CBC complex, NCBP2/CBP20 recognizes and binds capped RNAs (m7GpppG-capped RNA) but requires NCBP1/CBP80 to stabilize the movement of its N-terminal loop and lock the CBC into a high affinity cap-binding state with the cap structure. The conventional cap-binding complex with NCBP2 binds both small nuclear RNA (snRNA) and messenger (mRNA) and is involved in their export from the nucleus (PubMed:26382858).
Gene: NCBP2
-
分子量
- 18 kDa
-
基因ID
- 22916
-
UniProt
- P52298
-
途径
- Ribonucleoprotein Complex Subunit Organization, Methionine Biosynthetic Process
抗原
-
