POFUT1 抗体 (C-Term)
Quick Overview for POFUT1 抗体 (C-Term) (ABIN6257766)
抗原
See all POFUT1 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- C-Term
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特异性
- POFUT1 Antibody detects endogenous levels of total POFUT1.
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预测反应
- Pig,Bovine,Horse,Sheep,Dog,Chicken,Xenopus
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纯化方法
- The antiserum was purified by peptide affinity chromatography using SulfoLinkTM Coupling Resin (Thermo Fisher Scientific).
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免疫原
- A synthesized peptide derived from human POFUT1, corresponding to a region within C-terminal amino acids.
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亚型
- IgG
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anti-Protein O-Fucosyltransferase 1 (POFUT1) (AA 238-388) antibody
POFUT1 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Protein O-Fucosyltransferase 1 (POFUT1) (AA 1-194) antibodyPOFUT1 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Protein O-Fucosyltransferase 1 (POFUT1) (AA 243-393) antibodyPOFUT1 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Protein O-Fucosyltransferase 1 (POFUT1) antibodyPOFUT1 适用: 人, 小鼠, 大鼠 IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Protein O-Fucosyltransferase 1 (POFUT1) (AA 85-194) antibodyPOFUT1 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 3H1 unconjugated
anti-Protein O-Fucosyltransferase 1 (POFUT1) (C-Term) antibodyPOFUT1 适用: 人, 犬, 猴, Pig WB, IHC, IP, IF, IC 宿主: 兔 Polyclonal unconjugated
anti-Protein O-Fucosyltransferase 1 (POFUT1) (N-Term) antibodyPOFUT1 适用: 人, 小鼠 WB, IHC (p), EIA 宿主: 兔 Polyclonal unconjugated
anti-Protein O-Fucosyltransferase 1 (POFUT1) (AA 101-150) antibodyPOFUT1 适用: 人, 小鼠, 大鼠, 犬, 猴, Pig, Cow, 豚鼠, 马, 兔, 斑马鱼, Bat, Hamster WB 宿主: 兔 Polyclonal unconjugated
anti-Protein O-Fucosyltransferase 1 (POFUT1) (AA 344-373), (C-Term) antibodyPOFUT1 适用: 人 WB, IHC (p) 宿主: 兔 Polyclonal RB32435 unconjugated
anti-Protein O-Fucosyltransferase 1 (POFUT1) (Middle Region) antibodyPOFUT1 适用: 人, 小鼠, 大鼠, 犬, Cow, 豚鼠, 马, 兔, 斑马鱼 WB 宿主: 兔 Polyclonal unconjugated
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应用备注
- WB 1:500-1:1000, IHC: 1:50-1:200, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1 mg/mL
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缓冲液
- Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- Store at -20 °C. Stable for 12 months from date of receipt.
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有效期
- 12 months
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- POFUT1 (Protein O-Fucosyltransferase 1 (POFUT1))
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别名
- POFUT1
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背景
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Description: Catalyzes the reaction that attaches fucose through an O-glycosidic linkage to a conserved serine or threonine residue found in the consensus sequence C2-X(4,5)-[S/T]-C3 of EGF domains, where C2 and C3 are the second and third conserved cysteines. Specifically uses GDP-fucose as donor substrate and proper disulfide pairing of the substrate EGF domains is required for fucose transfer. Plays a crucial role in NOTCH signaling. Initial fucosylation of NOTCH by POFUT1 generates a substrate for FRINGE/RFNG, an acetylglucosaminyltransferase that can then extend the fucosylation on the NOTCH EGF repeats. This extended fucosylation is required for optimal ligand binding and canonical NOTCH signaling induced by DLL1 or JAGGED1. Fucosylates AGRN and determines its ability to cluster acetylcholine receptors (AChRs).
Gene: POFUT1
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分子量
- 44 kDa
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基因ID
- 23509
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UniProt
- Q9H488
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途径
- Notch Signaling, SARS-CoV-2 Protein Interactome
抗原
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