RNF111 抗体 (C-Term)
Quick Overview for RNF111 抗体 (C-Term) (ABIN6257485)
抗原
See all RNF111 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- C-Term
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特异性
- RNF111 Antibody detects endogenous levels of total RNF111.
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预测反应
- Pig,Bovine,Horse,Sheep,Rabbit,Dog,Chicken
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纯化方法
- The antiserum was purified by peptide affinity chromatography using SulfoLinkTM Coupling Resin (Thermo Fisher Scientific).
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免疫原
- A synthesized peptide derived from human RNF111, corresponding to a region within C-terminal amino acids.
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亚型
- IgG
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anti-Ring Finger Protein 111 (RNF111) (AA 1-108) antibody
RNF111 适用: 人 WB, ELISA, IF, RNAi 宿主: 小鼠 Monoclonal 1C4 unconjugated
anti-Ring Finger Protein 111 (RNF111) (AA 1-985) antibodyRNF111 适用: 人 WB, IHC (p) 宿主: 小鼠 Polyclonal unconjugated
anti-Ring Finger Protein 111 (RNF111) antibodyRNF111 适用: 人 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Ring Finger Protein 111 (RNF111) (AA 1-985) antibodyRNF111 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
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应用备注
- WB 1:500-1:1000, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1 mg/mL
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缓冲液
- Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- Store at -20 °C. Stable for 12 months from date of receipt.
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有效期
- 12 months
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- RNF111 (Ring Finger Protein 111 (RNF111))
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别名
- RNF111
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背景
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Description: E3 ubiquitin-protein ligase (PubMed:26656854). Required for mesoderm patterning during embryonic development (By similarity). Acts as an enhancer of the transcriptional responses of the SMAD2/SMAD3 effectors, which are activated downstream of BMP (PubMed:14657019, PubMed:16601693). Acts by mediating ubiquitination and degradation of SMAD inhibitors such as SMAD7, inducing their proteasomal degradation and thereby enhancing the transcriptional activity of TGF-beta and BMP (PubMed:14657019, PubMed:16601693). In addition to enhance transcription of SMAD2/SMAD3 effectors, also regulates their turnover by mediating their ubiquitination and subsequent degradation, coupling their activation with degradation, thereby ensuring that only effectors 'in use' are degraded (By similarity). Activates SMAD3/SMAD4-dependent transcription by triggering signal-induced degradation of SNON isoform of SKIL (PubMed:17591695). Associates with UBE2D2 as an E2 enzyme (PubMed:22411132). Specifically binds polysumoylated chains via SUMO interaction motifs (SIMs) and mediates ubiquitination of sumoylated substrates (PubMed:23751493). Catalyzes 'Lys-63'-linked ubiquitination of sumoylated XPC in response to UV irradiation, promoting nucleotide excision repair (PubMed:23751493). Mediates ubiquitination and degradation of sumoylated PML (By similarity). The regulation of the BMP-SMAD signaling is however independent of sumoylation and is not dependent of SUMO interaction motifs (SIMs) (By similarity).
Gene: RNF111
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分子量
- 109 kDa
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基因ID
- 54778
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UniProt
- Q6ZNA4
抗原
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