RFWD3 抗体 (N-Term)
Quick Overview for RFWD3 抗体 (N-Term) (ABIN6257465)
抗原
See all RFWD3 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- N-Term
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特异性
- RFWD3 Antibody detects endogenous levels of total RFWD3.
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纯化方法
- The antiserum was purified by peptide affinity chromatography using SulfoLinkTM Coupling Resin (Thermo Fisher Scientific).
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免疫原
- A synthesized peptide derived from human RFWD3, corresponding to a region within N-terminal amino acids.
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亚型
- IgG
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anti-Ring Finger and WD Repeat Domain 3 (RFWD3) (Internal Region) antibody
RFWD3 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Ring Finger and WD Repeat Domain 3 (RFWD3) (AA 651-749) antibodyRFWD3 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 6B4 unconjugated
anti-Ring Finger and WD Repeat Domain 3 (RFWD3) (Internal Region) antibodyRFWD3 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
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应用备注
- WB 1:500-1:1000, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1 mg/mL
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缓冲液
- Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- Store at -20 °C. Stable for 12 months from date of receipt.
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有效期
- 12 months
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- RFWD3 (Ring Finger and WD Repeat Domain 3 (RFWD3))
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别名
- RFWD3
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背景
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Description: E3 ubiquitin-protein ligase required for the repair of DNA interstrand cross-links (ICL) in response to DNA damage (PubMed:21504906, PubMed:21558276, PubMed:26474068, PubMed:28575657, PubMed:28575658). Plays a key role in RPA-mediated DNA damage signaling and repair (PubMed:21504906, PubMed:21558276, PubMed:26474068, PubMed:28575657, PubMed:28575658). Acts by mediating ubiquitination of the RPA complex (RPA1, RPA2 and RPA3 subunits) and RAD51 at stalled replication forks, leading to remove them from DNA damage sites and promote homologous recombination (PubMed:26474068, PubMed:28575657, PubMed:28575658). Also mediates the ubiquitination of p53/TP53 in the late response to DNA damage, and acts as a positive regulator of p53/TP53 stability, thereby regulating the G1/S DNA damage checkpoint (PubMed:20173098). May act by catalyzing the formation of short polyubiquitin chains on p53/TP53 that are not targeted to the proteasome (PubMed:20173098). In response to ionizing radiation, interacts with MDM2 and enhances p53/TP53 ubiquitination, possibly by restricting MDM2 from extending polyubiquitin chains on ubiquitinated p53/TP53 (PubMed:20173098).
Gene: RFWD3
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分子量
- 85 kDa
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基因ID
- 55159
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UniProt
- Q6PCD5
抗原
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