RAD1 抗体 (Internal Region)
Quick Overview for RAD1 抗体 (Internal Region) (ABIN6257451)
抗原
See all RAD1 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- Internal Region
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特异性
- RAD1 Antibody detects endogenous levels of total RAD1.
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预测反应
- Pig,Bovine,Horse,Sheep,Rabbit,Dog,Chicken,Xenopus
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纯化方法
- The antiserum was purified by peptide affinity chromatography using SulfoLinkTM Coupling Resin (Thermo Fisher Scientific).
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免疫原
- A synthesized peptide derived from human RAD1, corresponding to a region within the internal amino acids.
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亚型
- IgG
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anti-Cell Cycle Checkpoint Protein RAD1 (RAD1) (AA 1-90) antibody
RAD1 适用: 人 WB, ELISA, IHC (p), RNAi 宿主: 小鼠 Monoclonal 1G2 unconjugated
anti-Cell Cycle Checkpoint Protein RAD1 (RAD1) antibodyRAD1 适用: 人, 小鼠, 大鼠 IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Cell Cycle Checkpoint Protein RAD1 (RAD1) (AA 1-282) antibodyRAD1 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Cell Cycle Checkpoint Protein RAD1 (RAD1) (AA 1-282) antibodyRAD1 适用: 人 WB, IF 宿主: 小鼠 Polyclonal unconjugated
anti-Cell Cycle Checkpoint Protein RAD1 (RAD1) (AA 179-228) antibodyRAD1 适用: 人, Cow, 犬, 豚鼠, 马, 兔, Bat, Hamster, 猴, Pig WB 宿主: 兔 Polyclonal unconjugated
anti-Cell Cycle Checkpoint Protein RAD1 (RAD1) (Middle Region) antibodyRAD1 适用: 人, 小鼠, 大鼠, Cow, 犬, 豚鼠, 马, 兔 WB 宿主: 兔 Polyclonal unconjugated
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应用备注
- WB 1:500-1:2000, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1 mg/mL
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缓冲液
- Rabbit IgG in phosphate buffered saline, pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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有效期
- 12 months
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- RAD1 (Cell Cycle Checkpoint Protein RAD1 (RAD1))
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别名
- RAD1
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背景
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Description: Component of the 9-1-1 cell-cycle checkpoint response complex that plays a major role in DNA repair (PubMed:10846170, PubMed:10884395). The 9-1-1 complex is recruited to DNA lesion upon damage by the RAD17-replication factor C (RFC) clamp loader complex (PubMed:12578958). Acts then as a sliding clamp platform on DNA for several proteins involved in long-patch base excision repair (LP-BER) (PubMed:15871698). The 9-1-1 complex stimulates DNA polymerase beta (POLB) activity by increasing its affinity for the 3'-OH end of the primer-template and stabilizes POLB to those sites where LP-BER proceeds, endonuclease FEN1 cleavage activity on substrates with double, nick, or gap flaps of distinct sequences and lengths, and DNA ligase I (LIG1) on long-patch base excision repair substrates (PubMed:15314187, PubMed:15556996, PubMed:15871698). The 9-1-1 complex is necessary for the recruitment of RHNO1 to sites of double-stranded breaks (DSB) occurring during the S phase (PubMed:21659603). Isoform 1 possesses 3'->5' double stranded DNA exonuclease activity (PubMed:9660799).
Gene: RAD1
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分子量
- 32 kDa
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基因ID
- 5810
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UniProt
- O60671
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途径
- M Phase
抗原
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