SAPK, JNK (pTyr185) 抗体
(1 reference)Quick Overview for SAPK, JNK (pTyr185) 抗体 (ABIN6256659)
抗原
适用
宿主
克隆类型
标记
应用范围
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抗原表位
- pTyr185
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特异性
- Phospho-SAPK/JNK (Tyr185) Antibody detects endogenous levels of SAPK/JNK only when phosphorylated at Tyrosine 185
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交叉反应
- 人, 小鼠, 大鼠
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纯化方法
- The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.
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免疫原
- A synthesized peptide derived from human SAPK/JNK around the phosphorylation site of Tyrosine 185
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亚型
- IgG
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anti-SAPK, JNK (pThr183) antibody
适用: 人, 小鼠, 大鼠 IHC, WB, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-SAPK, JNK (AA 166-215) antibody适用: 人, 小鼠, 大鼠 IHC, WB, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-SAPK, JNK (AA 151-200), (pTyr185) antibody适用: 人, 小鼠, 大鼠 IHC, WB, ELISA 宿主: 兔 Polyclonal unconjugated
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应用备注
- WB 1:500-1:2000 IHC 1:50-1:200, IF/ICC 1:100-1:500
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1 mg/mL
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缓冲液
- Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- Store at -20 °C.Stable for 12 months from date of receipt
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有效期
- 12 months
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: "Ficolin-A/2, acting as a new regulator of macrophage polarization, mediates the inflammatory response in experimental mouse colitis." in: Immunology, Vol. 151, Issue 4, pp. 433-450, (2017) (PubMed).
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: "Ficolin-A/2, acting as a new regulator of macrophage polarization, mediates the inflammatory response in experimental mouse colitis." in: Immunology, Vol. 151, Issue 4, pp. 433-450, (2017) (PubMed).
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- SAPK, JNK
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别名
- SAPK/JNK
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背景
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Description: Serine/threonine-protein kinase involved in various processes such as cell proliferation, differentiation, migration, transformation and programmed cell death. Extracellular stimuli such as proinflammatory cytokines or physical stress stimulate the stress-activated protein kinase/c-Jun N-terminal kinase (SAP/JNK) signaling pathway. In this cascade, two dual specificity kinases MAP2K4/MKK4 and MAP2K7/MKK7 phosphorylate and activate MAPK8/JNK1. In turn, MAPK8/JNK1 phosphorylates a number of transcription factors, primarily components of AP-1 such as JUN, JDP2 and ATF2 and thus regulates AP-1 transcriptional activity. Phosphorylates the replication licensing factor CDT1, inhibiting the interaction between CDT1 and the histone H4 acetylase HBO1 to replication origins. Loss of this interaction abrogates the acetylation required for replication initiation. Promotes stressed cell apoptosis by phosphorylating key regulatory factors including p53/TP53 and Yes-associates protein YAP1. In T-cells, MAPK8 and MAPK9 are required for polarized differentiation of T-helper cells into Th1 cells. Contributes to the survival of erythroid cells by phosphorylating the antagonist of cell death BAD upon EPO stimulation. Mediates starvation-induced BCL2 phosphorylation, BCL2 dissociation from BECN1, and thus activation of autophagy. Phosphorylates STMN2 and hence regulates microtubule dynamics, controlling neurite elongation in cortical neurons. In the developing brain, through its cytoplasmic activity on STMN2, negatively regulates the rate of exit from multipolar stage and of radial migration from the ventricular zone. Phosphorylates several other substrates including heat shock factor protein 4 (HSF4), the deacetylase SIRT1, ELK1, or the E3 ligase ITCH. Phosphorylates the CLOCK-ARNTL/BMAL1 heterodimer and plays a role in the regulation of the circadian clock (PubMed:22441692). Phosphorylates the heat shock transcription factor HSF1, suppressing HSF1-induced transcriptional activity (PubMed:10747973).
Gene: MAPK8
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分子量
- 45kDa
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基因ID
- 5599
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UniProt
- P45983, P45984, P53779
抗原
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