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Aurora Kinase B 抗体 (pThr232)

Name: Aurora Kinase B 抗体 (pThr232)
SKU: ABIN6256516
Availability: OutOfStock

This anti-Aurora Kinase B antibody is a 兔多克隆 antibody detecting Aurora Kinase B in WB, IHC, ELISA, IF 和 ICC. Suitable for 人, 小鼠, 大鼠和猴.

Western blot analysis of AurB phosphorylation expression in Nocodazole treated COS7 whole cell lysates,The lane on the left is treated with the antigen-specific peptide. (Aurora Kinase B 抗体 (pThr232))

ABIN6267684 at 1/100 staining human Bone marrow tissue sections by IHC-P. (Aurora Kinase B 抗体 (pThr232))

IHC

ABIN6267684 staining HT-1080 cells by ICC/IF. (Aurora Kinase B 抗体 (pThr232))

IF/ICC

4 images

产品编号 ABIN6256516

发货至: 中国

Datasheet as PDF

Quick Overview for Aurora Kinase B 抗体 (pThr232) (ABIN6256516)

抗原

See all Aurora Kinase B (AURKB) 抗体

Aurora Kinase B (AURKB)

适用

人, 小鼠, 大鼠, 猴

宿主

兔

克隆类型

多克隆

标记

This Aurora Kinase B antibody is un-conjugated

应用范围

Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC)

Discover our top product AURKB Primary Antibody

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pThr232

特异性

Phospho-AurB (Thr232) Antibody detects endogenous levels of AurB only when phosphorylated at Threonine 232

交叉反应

人, 猴, 小鼠, 大鼠

纯化方法

The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.

免疫原

A synthesized peptide derived from human AurB around the phosphorylation site of Threonine 232

亚型

IgG
anti-Aurora Kinase B (AURKB) (AA 1-90) antibody
AURKB 适用: 人 WB, ELISA, IF, IP 宿主: 小鼠 Monoclonal 6H7 unconjugated

anti-Aurora Kinase B (AURKB) (Internal Region) antibody
AURKB 适用: 人, 猴 WB, IHC, ELISA, FM 宿主: 兔 Polyclonal unconjugated

anti-Aurora Kinase B (AURKB) (AA 6-35) antibody
AURKB 适用: 人, 小鼠, 大鼠, 灵长类 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated

anti-Aurora Kinase B (AURKB) antibody
AURKB 适用: 人 WB, IHC, ELISA, IP 宿主: 兔 Monoclonal 4E5 unconjugated Recombinant Antibody

anti-Aurora Kinase B (AURKB) (AA 1-90) antibody
AURKB 适用: 人 WB, ELISA, RNAi 宿主: 小鼠 Monoclonal 6G8 unconjugated

anti-Aurora Kinase B (AURKB) (AA 89-251) antibody
AURKB 适用: 人 IHC, StM 宿主: 小鼠 Monoclonal rAURKB-1592 unconjugated Recombinant Antibody

anti-Aurora Kinase B (AURKB) (AA 1-255) antibody
AURKB 适用: 人 IHC, ELISA, IF 宿主: 兔 Polyclonal unconjugated

anti-Aurora Kinase B (AURKB) (pThr232) antibody
AURKB 适用: 人, 小鼠, 大鼠, Cow, Pig WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated

anti-Aurora Kinase B (AURKB) (AA 89-251) antibody
AURKB 适用: 人 IHC, StM 宿主: 小鼠 Monoclonal AURKB-1592 unconjugated

anti-Aurora Kinase B (AURKB) (AA 89-251) antibody
AURKB 适用: 人 IHC, StM 宿主: 兔 Monoclonal AURKB-3121R unconjugated Recombinant Antibody

应用备注

WB 1:500-1:2000 IHC 1:50-1:200 IF/ICC 1:100-1:500

限制

仅限研究用
状态

Liquid

浓度

1 mg/mL

缓冲液

Rabbit IgG in phosphate buffered saline ,  pH 7.4, 150  mM NaCl, 0.02 % sodium azide and 50 % glycerol.

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

-20 °C

储存方法

Store at -20 °C.Stable for 12 months from date of receipt

有效期

12 months
抗原

Aurora Kinase B (AURKB)

别名

AurB

背景

Description: Serine/threonine-protein kinase component of the chromosomal passenger complex (CPC), a complex that acts as a key regulator of mitosis. The CPC complex has essential functions at the centromere in ensuring correct chromosome alignment and segregation and is required for chromatin-induced microtubule stabilization and spindle assembly. Involved in the bipolar attachment of spindle microtubules to kinetochores and is a key regulator for the onset of cytokinesis during mitosis. Required for central/midzone spindle assembly and cleavage furrow formation. Key component of the cytokinesis checkpoint, a process required to delay abscission to prevent both premature resolution of intercellular chromosome bridges and accumulation of DNA damage: phosphorylates CHMP4C, leading to retain abscission-competent VPS4 (VPS4A and/or VPS4B) at the midbody ring until abscission checkpoint signaling is terminated at late cytokinesis (PubMed:22422861, PubMed:24814515). AURKB phosphorylates the CPC complex subunits BIRC5/survivin, CDCA8/borealin and INCENP. Phosphorylation of INCENP leads to increased AURKB activity. Other known AURKB substrates involved in centromeric functions and mitosis are CENPA, DES/desmin, GPAF, KIF2C, NSUN2, RACGAP1, SEPT1, VIM/vimentin, HASPIN, and histone H3. A positive feedback loop involving HASPIN and AURKB contributes to localization of CPC to centromeres. Phosphorylation of VIM controls vimentin filament segregation in cytokinetic process, whereas histone H3 is phosphorylated at 'Ser-10' and 'Ser-28' during mitosis (H3S10ph and H3S28ph, respectively). A positive feedback between HASPIN and AURKB contributes to CPC localization. AURKB is also required for kinetochore localization of BUB1 and SGO1. Phosphorylation of p53/TP53 negatively regulates its transcriptional activity. Key regulator of active promoters in resting B- and T-lymphocytes: acts by mediating phosphorylation of H3S28ph at active promoters in resting B-cells, inhibiting RNF2/RING1B-mediated ubiquitination of histone H2A and enhancing binding and activity of the USP16 deubiquitinase at transcribed genes.
Gene: AURKB

分子量

39kDa

基因ID

9212

UniProt

Q96GD4

途径

TCR Signaling, Cell Division Cycle, Maintenance of Protein Location, Hepatitis C, Toll-Like Receptors Cascades