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HSF1 抗体 (pSer303)

Name: HSF1 抗体 (pSer303)
SKU: ABIN6256259
Availability: OutOfStock

This anti-HSF1 antibody is a 兔多克隆 antibody detecting HSF1 in WB, ELISA, IHC, IF 和 ICC. Suitable for 人和小鼠.

Western blot analysis of HSF1 phosphorylation expression in TNF-α treated MCF7 whole cell lysates,The lane on the left is treated with the antigen-specific peptide. (HSF1 抗体 (pSer303))

ABIN6267581 at 1/100 staining human breast carcinoma tissue sections by IHC-P. (HSF1 抗体 (pSer303))

IHC

Western blot analysis of extracts from various samples, using Phospho-HSF1 (Ser303) Antibody. (HSF1 抗体 (pSer303))

Western blot analysis of Phospho-HSF1 (Ser303) expression in various lysates (HSF1 抗体 (pSer303))

4 images

产品编号 ABIN6256259

发货至: 中国

Quick Overview for HSF1 抗体 (pSer303) (ABIN6256259)

抗原

See all HSF1 抗体

HSF1 (Heat Shock Factor Protein 1 (HSF1))

适用

人, 小鼠

宿主

兔

克隆类型

多克隆

标记

This HSF1 antibody is un-conjugated

应用范围

Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)

Discover our top product HSF1 Primary Antibody

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pSer303

特异性

Phospho-HSF1 (Ser303) Antibody detects endogenous levels of HSF1 only when phosphorylated at Serine 303.

预测反应

Bovine,Horse

纯化方法

The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.

免疫原

A synthesized peptide derived from human HSF1 around the phosphorylation site of Ser303.

亚型

IgG
anti-Heat Shock Factor Protein 1 (HSF1) (AA 378-395) antibody (HRP)
HSF1 适用: 小鼠 WB, ELISA, IHC, IP, IF, GS, ICC 宿主: 大鼠 Monoclonal 10H8 HRP

anti-Heat Shock Factor Protein 1 (HSF1) (AA 378-395) antibody (FITC)
HSF1 适用: 小鼠 WB, ELISA, IHC, IP, IF, GS, ICC 宿主: 大鼠 Monoclonal 10H8 FITC

anti-Heat Shock Factor Protein 1 (HSF1) (AA 378-395) antibody (Biotin)
HSF1 适用: 小鼠 WB, ELISA, IHC, IP, IF, GS, ICC 宿主: 大鼠 Monoclonal 10H8 Biotin

anti-Heat Shock Factor Protein 1 (HSF1) (AA 378-395) antibody (PE)
HSF1 适用: 小鼠 WB, ELISA, IHC, IP, IF, GS, ICC 宿主: 大鼠 Monoclonal 10H8 PE

anti-Heat Shock Factor Protein 1 (HSF1) (AA 378-395) antibody (APC)
HSF1 适用: 小鼠 WB, ELISA, IHC, IP, IF, GS, ICC 宿主: 大鼠 Monoclonal 10H8 APC

anti-Heat Shock Factor Protein 1 (HSF1) (AA 378-395) antibody (PerCP)
HSF1 适用: 小鼠 WB, ELISA, IHC, IP, IF, GS, ICC 宿主: 大鼠 Monoclonal 10H8 PerCP

anti-Heat Shock Factor Protein 1 (HSF1) (AA 378-395) antibody (Atto 488)
HSF1 适用: 小鼠 WB, ELISA, IHC, IP, IF, GS, ICC 宿主: 大鼠 Monoclonal 10H8 Atto 488

anti-Heat Shock Factor Protein 1 (HSF1) (AA 378-395) antibody (Atto 594)
HSF1 适用: 小鼠 WB, ELISA, IHC, IP, IF, GS, ICC 宿主: 大鼠 Monoclonal 10H8 Atto 594

anti-Heat Shock Factor Protein 1 (HSF1) (AA 378-395) antibody (Atto 390)
HSF1 适用: 小鼠 WB, ELISA, IHC, IP, IF, GS, ICC 宿主: 大鼠 Monoclonal 10H8 Atto 390

anti-Heat Shock Factor Protein 1 (HSF1) (AA 350-529) antibody
HSF1 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated

应用备注

WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000

限制

仅限研究用
状态

Liquid

浓度

1 mg/mL

缓冲液

Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

-20 °C

储存方法

Store at -20 °C. Stable for 12 months from date of receipt.

有效期

12 months
抗原

HSF1 (Heat Shock Factor Protein 1 (HSF1))

别名

HSF1

背景

Description: Function as a stress-inducible and DNA-binding transcription factor that plays a central role in the transcriptional activation of the heat shock response (HSR), leading to the expression of a large class of molecular chaperones heat shock proteins (HSPs) that protect cells from cellular insults' damage (PubMed:1871105, PubMed:11447121, PubMed:1986252, PubMed:7760831, PubMed:7623826, PubMed:8946918, PubMed:8940068, PubMed:9341107, PubMed:9121459, PubMed:9727490, PubMed:9499401, PubMed:9535852, PubMed:12659875, PubMed:12917326, PubMed:15016915, PubMed:25963659, PubMed:26754925). In unstressed cells, is present in a HSP90-containing multichaperone complex that maintains it in a non-DNA-binding inactivated monomeric form (PubMed:9727490, PubMed:11583998, PubMed:16278218). Upon exposure to heat and other stress stimuli, undergoes homotrimerization and activates HSP gene transcription through binding to site-specific heat shock elements (HSEs) present in the promoter regions of HSP genes (PubMed:1871105, PubMed:1986252, PubMed:8455624, PubMed:7935471, PubMed:7623826, PubMed:8940068, PubMed:9727490, PubMed:9499401, PubMed:10359787, PubMed:11583998, PubMed:12659875, PubMed:16278218, PubMed:25963659, PubMed:26754925). Activation is reversible, and during the attenuation and recovery phase period of the HSR, returns to its unactivated form (PubMed:11583998, PubMed:16278218). Binds to inverted 5'-NGAAN-3' pentamer DNA sequences (PubMed:1986252, PubMed:26727489). Binds to chromatin at heat shock gene promoters (PubMed:25963659). Plays also several other functions independently of its transcriptional activity. Involved in the repression of Ras-induced transcriptional activation of the c-fos gene in heat-stressed cells (PubMed:9341107). Positively regulates pre-mRNA 3'-end processing and polyadenylation of HSP70 mRNA upon heat-stressed cells in a symplekin (SYMPK)-dependent manner (PubMed:14707147). Plays a role in nuclear export of stress-induced HSP70 mRNA (PubMed:17897941). Plays a role in the regulation of mitotic progression (PubMed:18794143). Plays also a role as a negative regulator of non-homologous end joining (NHEJ) repair activity in a DNA damage-dependent manner (PubMed:26359349). Involved in stress-induced cancer cell proliferation in a IER5-dependent manner (PubMed:26754925).
Gene: HSF1

分子量

82kDa

基因ID

3297

UniProt

Q00613