HSF1 抗体 (pSer303)
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- 抗原 See all HSF1 抗体
- HSF1 (Heat Shock Factor Protein 1 (HSF1))
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抗原表位
- pSer303
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适用
- 人, 小鼠
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This HSF1 antibody is un-conjugated
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应用范围
- Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)
- 特异性
- Phospho-HSF1 (Ser303) Antibody detects endogenous levels of HSF1 only when phosphorylated at Serine 303.
- 预测反应
- Bovine,Horse
- 纯化方法
- The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.
- 免疫原
- A synthesized peptide derived from human HSF1 around the phosphorylation site of Ser303.
- 亚型
- IgG
- Top Product
- Discover our top product HSF1 Primary Antibody
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anti-Heat Shock Factor Protein 1 (HSF1) (AA 378-395) antibody (Alkaline Phosphatase (AP))
HSF1 适用: 小鼠 WB, ELISA, IHC, IP, IF, GS, ICC 宿主: 大鼠 Monoclonal 10H8 Alkaline Phosphatase (AP)
anti-Heat Shock Factor Protein 1 (HSF1) (AA 378-395) antibody (HRP)HSF1 适用: 小鼠 WB, ELISA, IHC, IP, IF, GS, ICC 宿主: 大鼠 Monoclonal 10H8 HRP
anti-Heat Shock Factor Protein 1 (HSF1) (AA 378-395) antibody (FITC)HSF1 适用: 小鼠 WB, ELISA, IHC, IP, IF, GS, ICC 宿主: 大鼠 Monoclonal 10H8 FITC
anti-Heat Shock Factor Protein 1 (HSF1) (AA 378-395) antibody (APC)HSF1 适用: 小鼠 WB, ELISA, IHC, IP, IF, GS, ICC 宿主: 大鼠 Monoclonal 10H8 APC
anti-Heat Shock Factor Protein 1 (HSF1) (AA 378-395) antibody (Biotin)HSF1 适用: 小鼠 WB, ELISA, IHC, IP, IF, GS, ICC 宿主: 大鼠 Monoclonal 10H8 Biotin
anti-Heat Shock Factor Protein 1 (HSF1) (AA 378-395) antibody (PE)HSF1 适用: 小鼠 WB, ELISA, IHC, IP, IF, GS, ICC 宿主: 大鼠 Monoclonal 10H8 PE
anti-Heat Shock Factor Protein 1 (HSF1) (AA 378-395) antibody (PerCP)HSF1 适用: 小鼠 WB, ELISA, IHC, IP, IF, GS, ICC 宿主: 大鼠 Monoclonal 10H8 PerCP
anti-Heat Shock Factor Protein 1 (HSF1) (AA 378-395) antibody (Atto 488)HSF1 适用: 小鼠 WB, ELISA, IHC, IP, IF, GS, ICC 宿主: 大鼠 Monoclonal 10H8 Atto 488
anti-Heat Shock Factor Protein 1 (HSF1) (AA 378-395) antibody (Atto 594)HSF1 适用: 小鼠 WB, ELISA, IHC, IP, IF, GS, ICC 宿主: 大鼠 Monoclonal 10H8 Atto 594
anti-Heat Shock Factor Protein 1 (HSF1) (AA 378-395) antibody (Atto 390)HSF1 适用: 小鼠 WB, ELISA, IHC, IP, IF, GS, ICC 宿主: 大鼠 Monoclonal 10H8 Atto 390
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- 应用备注
- WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 1 mg/mL
- 缓冲液
- Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- -20 °C
- 储存方法
- Store at -20 °C. Stable for 12 months from date of receipt.
- 有效期
- 12 months
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- 抗原
- HSF1 (Heat Shock Factor Protein 1 (HSF1))
- 别名
- HSF1 (HSF1 产品)
- 背景
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Description: Function as a stress-inducible and DNA-binding transcription factor that plays a central role in the transcriptional activation of the heat shock response (HSR), leading to the expression of a large class of molecular chaperones heat shock proteins (HSPs) that protect cells from cellular insults' damage (PubMed:1871105, PubMed:11447121, PubMed:1986252, PubMed:7760831, PubMed:7623826, PubMed:8946918, PubMed:8940068, PubMed:9341107, PubMed:9121459, PubMed:9727490, PubMed:9499401, PubMed:9535852, PubMed:12659875, PubMed:12917326, PubMed:15016915, PubMed:25963659, PubMed:26754925). In unstressed cells, is present in a HSP90-containing multichaperone complex that maintains it in a non-DNA-binding inactivated monomeric form (PubMed:9727490, PubMed:11583998, PubMed:16278218). Upon exposure to heat and other stress stimuli, undergoes homotrimerization and activates HSP gene transcription through binding to site-specific heat shock elements (HSEs) present in the promoter regions of HSP genes (PubMed:1871105, PubMed:1986252, PubMed:8455624, PubMed:7935471, PubMed:7623826, PubMed:8940068, PubMed:9727490, PubMed:9499401, PubMed:10359787, PubMed:11583998, PubMed:12659875, PubMed:16278218, PubMed:25963659, PubMed:26754925). Activation is reversible, and during the attenuation and recovery phase period of the HSR, returns to its unactivated form (PubMed:11583998, PubMed:16278218). Binds to inverted 5'-NGAAN-3' pentamer DNA sequences (PubMed:1986252, PubMed:26727489). Binds to chromatin at heat shock gene promoters (PubMed:25963659). Plays also several other functions independently of its transcriptional activity. Involved in the repression of Ras-induced transcriptional activation of the c-fos gene in heat-stressed cells (PubMed:9341107). Positively regulates pre-mRNA 3'-end processing and polyadenylation of HSP70 mRNA upon heat-stressed cells in a symplekin (SYMPK)-dependent manner (PubMed:14707147). Plays a role in nuclear export of stress-induced HSP70 mRNA (PubMed:17897941). Plays a role in the regulation of mitotic progression (PubMed:18794143). Plays also a role as a negative regulator of non-homologous end joining (NHEJ) repair activity in a DNA damage-dependent manner (PubMed:26359349). Involved in stress-induced cancer cell proliferation in a IER5-dependent manner (PubMed:26754925).
Gene: HSF1
- 分子量
- 82kDa
- 基因ID
- 3297
- UniProt
- Q00613
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