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CD28 抗体 (pTyr218)

This anti-CD28 antibody is a 兔 多克隆 antibody detecting CD28 in WB, IF, ELISA 和 ICC. Suitable for 人, 小鼠 和 大鼠.
产品编号 ABIN6255852
发货至: 中国

Quick Overview for CD28 抗体 (pTyr218) (ABIN6255852)

抗原

See all CD28 抗体
CD28

适用

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人, 小鼠, 大鼠

宿主

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克隆类型

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多克隆

标记

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This CD28 antibody is un-conjugated

应用范围

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Western Blotting (WB), Immunofluorescence (IF), ELISA, Immunocytochemistry (ICC)
  • 抗原表位

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    pTyr218

    特异性

    Phospho-CD28 (Tyr218) Antibody detects endogenous levels of CD28 only when phosphorylated at Tyr218.

    预测反应

    Pig,Bovine,Horse,Sheep,Rabbit,Dog

    纯化方法

    The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.

    免疫原

    A synthesized peptide derived from human CD28 around the phosphorylation site of Tyr218.

    亚型

    IgG
  • 应用备注

    WB 1:1000-3000, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    1 mg/mL

    缓冲液

    Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    储存条件

    -20 °C

    储存方法

    Store at -20 °C. Stable for 12 months from date of receipt.

    有效期

    12 months
  • 抗原

    CD28

    别名

    CD28

    背景

    Description: Involved in T-cell activation, the induction of cell proliferation and cytokine production and promotion of T-cell survival. Enhances the production of IL4 and IL10 in T-cells in conjunction with TCR/CD3 ligation and CD40L costimulation (PubMed:8617933). Isoform 3 enhances CD40L-mediated activation of NF-kappa-B and kinases MAPK8 and PAK2 in T-cells (PubMed:15067037).

    Gene: CD28

    分子量

    25 kDa

    基因ID

    940

    UniProt

    P10747

    途径

    TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response
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