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FMR1 抗体 (pSer499)

Name: FMR1 抗体 (pSer499)
Brand: antibodies-online
SKU: ABIN6254033
Availability: OutOfStock

This anti-FMR1 antibody is a 兔多克隆 antibody detecting FMR1 in WB, IHC 和 ELISA. Suitable for 大鼠. This Primary Antibody has been cited in 4+ publications.

Western blot of rat hippocampal lysate showing specific immunolabeling of the ~71 kDa FMRP protein phosphorylated at Ser499 in the first lane (-). (FMR1 抗体 (pSer499))

1 image

(4 references)

产品编号 ABIN6254033

发货至: 中国

Quick Overview for FMR1 抗体 (pSer499) (ABIN6254033)

抗原

See all FMR1 抗体

FMR1 (Fragile X Mental Retardation 1 (FMR1))

适用

大鼠

宿主

兔

克隆类型

多克隆

标记

This FMR1 antibody is un-conjugated

应用范围

Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Discover our top product FMR1 Primary Antibody

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pSer499

特异性

Specific for endogenous levels of the ~71 kDa FMRP protein phosphorylated at Ser499. Immunolabeling is completely eliminated by treatment with λ-phosphatase.

交叉反应

小鸡, 人, 小鼠, 大鼠

预测反应

bovine, canine, chicken, human, mouse, non-human primates, sheep, Xenopus, zebra fish

产品特性

Our FMRP (Ser499) rabbit polyclonal phosphospecific primary antibody is produced in-house. It detects FMRP (Ser499) and is antigen affinity purified from pooled serum. It is great for use in ELISA, WB, IHC.

纯化方法

Prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho and non-phosphopeptide affinity columns.

免疫原

Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser499 of rat FMRP, conjugated to keyhole limpet hemocyanin (KLH).

亚型

IgG
anti-Fragile X Mental Retardation 1 (FMR1) (AA 36-279) antibody
FMR1 适用: 人, 小鼠, 大鼠, 犬, 猴 WB, IHC, IF, IHC (p), FACS 宿主: 小鼠 Monoclonal 1C6 unconjugated

anti-Fragile X Mental Retardation 1 (FMR1) (AA 26-279) antibody
FMR1 适用: 人, 小鼠, 大鼠, 犬, 猴 WB, IHC, IHC (p) 宿主: 小鼠 Monoclonal 2C3 unconjugated

anti-Fragile X Mental Retardation 1 (FMR1) antibody
FMR1 适用: 人 WB, IHC, IF, IHC (p), FACS 宿主: 小鼠 Monoclonal 3B4 unconjugated

anti-Fragile X Mental Retardation 1 (FMR1) (AA 121-220) antibody
FMR1 适用: 人 WB, ELISA, RNAi 宿主: 小鼠 Monoclonal 2D4 unconjugated

anti-Fragile X Mental Retardation 1 (FMR1) (AA 500-600) antibody
FMR1 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated

anti-Fragile X Mental Retardation 1 (FMR1) (AA 116-130) antibody
Verified FMR1 适用: 人 WB, IHC, ELISA 宿主: 山羊 Polyclonal unconjugated

anti-Fragile X Mental Retardation 1 (FMR1) (AA 121-220) antibody
FMR1 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 3E11 unconjugated

anti-Fragile X Mental Retardation 1 (FMR1) antibody
FMR1 适用: 人 IHC, ELISA, ICC 宿主: 小鼠 Monoclonal 4G9 unconjugated

anti-Fragile X Mental Retardation 1 (FMR1) (AA 20-48), (N-Term) antibody
FMR1 适用: 人, 小鼠 WB, IHC (p), FACS 宿主: 兔 Polyclonal RB20964 unconjugated

anti-Fragile X Mental Retardation 1 (FMR1) (pSer500) antibody
FMR1 适用: 人, 小鼠, 大鼠, 斑马鱼 WB, ELISA, IHC (p), ICC, IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated

应用备注

Dilution Range: WB Brain: 1:1000
Dilution Range: WB: 1:1000

限制

仅限研究用
状态

Liquid

缓冲液

10 mM HEPES ( pH 7.5), 150 mM NaCl, 100 μg per ml BSA and 50 % glycerol.

储存条件

-20 °C

储存方法

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

有效期

12 months
Bartley, OKeefe, Blice-Baum, Mihailescu, Gong, Miyares, Karaca, Bordey: "Mammalian FMRP S499 Is Phosphorylated by CK2 and Promotes Secondary Phosphorylation of FMRP." in: eNeuro, Vol. 3, Issue 6, (2017) (PubMed).

Reynolds, Smith, Jefferson, Lugo: "Comparison of Equivalence between Two Commercially Available S499-Phosphorylated FMRP Antibodies in Mice." in: PLoS ONE, Vol. 10, Issue 11, pp. e0143134, (2016) (PubMed).

Guimarães-Souza, Perche, Morgans, Duvoisin, Calaza: "Fragile X Mental Retardation Protein expression in the retina is regulated by light." in: Experimental eye research, Vol. 146, pp. 72-82, (2016) (PubMed).

Bartley, OKeefe, Bordey: "FMRP S499 is phosphorylated independent of mTORC1-S6K1 activity." in: PLoS ONE, Vol. 9, Issue 5, pp. e96956, (2015) (PubMed).
抗原

FMR1 (Fragile X Mental Retardation 1 (FMR1))

别名

FMR1

背景

Synonyms: FMR 1 antibody, Fmr1 antibody, Fmr1 gene antibody, FMR1_HUMAN antibody, FMRP antibody, Fragile X mental retardation 1 antibody, Fragile X mental retardation 1 protein antibody, Fragile X mental retardation protein 1 antibody, Fragile X mental retardation protein antibody, fragile X mental retardation syndrome-related protein 1 antibody, fragile X mental retardation autosomal homolog 1 antibody, FRAXA antibody, fxr1 antibody, MGC87458 antibody, POF antibody, POF1 antibody, Protein FMR-1 antibody, Protein FMR1 antibody, wu:fb16f11 antibody, wu:fd18c10 antibody, zgc:66226 antibody
Description Logic: Our FMRP (Ser499) rabbit polyclonal phosphospecific primary antibody is produced . It detects FMRP (Ser499) and is antigen affinity purified from pooled serum. It is great for use in ELISA, WB, IHC.
Gene Name Alternatives: FMR1

分子量

71 kDa

基因ID

24948

UniProt

Q80WE1

途径

Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development