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CAMK2A 抗体 (pThr306)

This anti-CAMK2A antibody is a 兔 多克隆 antibody detecting CAMK2A in WB 和 ELISA. Suitable for 大鼠. This Primary Antibody has been cited in 1 publication.
产品编号 ABIN6254013
发货至: 中国

Quick Overview for CAMK2A 抗体 (pThr306) (ABIN6254013)

抗原

See all CAMK2A 抗体
CAMK2A (Calcium/calmodulin-Dependent Protein Kinase II alpha (CAMK2A))

适用

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大鼠

宿主

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克隆类型

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多克隆

标记

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This CAMK2A antibody is un-conjugated

应用范围

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Western Blotting (WB), ELISA
  • 抗原表位

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    pThr306

    特异性

    Specific for endogenous levels of the ~50 kDa α-CaM Kinase II and the ~60 kDa β-CaM Kinase II proteins phosphorylated at Thr306. Immunolabeling is completely eliminated by treatment with λ-phosphatase. It has been reported that this antibody may recognize some level of non-phosphorylated pure recombinant protein, but in our hands (as shown in our WB image) in native tissue, the antibody reacts in a phospho-specific manner.

    交叉反应

    小鼠, 大鼠

    预测反应

    bovine, canine, chicken, human, Xenopus, Zebrafish

    产品特性

    Our CaM Kinase II (Thr306) rabbit polyclonal phosphospecific primary antibody is produced in-house. It detects CaM Kinase II (Thr306) and is antigen affinity purified from pooled serum. It is great for use in ELISA, WB.

    纯化方法

    Prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho and non-phosphopeptide affinity columns.

    免疫原

    Synthetic phospho-peptide corresponding to amino acid residues surrounding Thr306 of rat CaMKII, conjugated to keyhole limpet hemocyanin (KLH).

    亚型

    IgG
  • 应用备注

    Dilution Range: WB Brain: 1:1000

    Dilution Range: WB: 1:1000

    限制

    仅限研究用
  • 状态

    Liquid

    缓冲液

    10 mM HEPES ( pH 7.5), 150 mM NaCl, 100 μg per ml BSA and 50 % glycerol.

    储存条件

    -20 °C

    储存方法

    Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

    有效期

    12 months
  • Bhattacharyya, Lee, Muratcioglu, Qiu, Nyayapati, Schulman, Groves, Kuriyan: "Flexible linkers in CaMKII control the balance between activating and inhibitory autophosphorylation." in: eLife, Vol. 9, (2020) (PubMed).

  • 抗原

    CAMK2A (Calcium/calmodulin-Dependent Protein Kinase II alpha (CAMK2A))

    别名

    CaM Kinase II

    背景

    Synonyms: Calcium/calmodulin dependent protein kinase II alpha antibody, Calcium/calmodulin dependent protein kinase II beta antibody, Calcium/calmodulin dependent protein kinase II delta antibody, Calcium/calmodulin dependent protein kinase II gamma antibody, Calcium/calmodulin-dependent protein kinase type II subunit alpha antibody, CaM kinase II alpha antibody, CaM kinase II antibody, CaM kinase II beta antibody, CaM kinase II delta antibody, CaM kinase II gamma antibody, CaM kinase II subunit alpha antibody, CaMK-II subunit alpha antibody, CAMK2 antibody, Camk2a antibody, CAMK2B antibody, CAMK2D antibody, CAMK2G antibody, CAMKA antibody, KCC2A_HUMAN antibody

    Description Logic: Our CaM Kinase II (Thr306) rabbit polyclonal phosphospecific primary antibody is produced . It detects CaM Kinase II (Thr306) and is antigen affinity purified from pooled serum. It is great for use in ELISA, WB.

    Gene Name Alternatives: CAMK2A/B

    分子量

    50 kDa/60 kDa

    基因ID

    25400

    UniProt

    P11310

    途径

    WNT signaling, Interferon-gamma Pathway, Myometrial Relaxation and Contraction
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