Tubulin 抗体
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- 抗原 See all Tubulin (TUB) 抗体
- Tubulin (TUB)
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适用
- 人, 小鼠, 犬
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This Tubulin antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunocytochemistry (ICC), Immunoprecipitation (IP)
- 原理
- anti-Tubulin (glycylated), pAb (Gly-pep1)
- 产品特性
- Polyclonal Antibody. This antibody recognizes mono or bi-glycylated Tubulins. The activity of glycylating enzymes (TTLL3 and TTLL8) in cultured cells leads mainly to the modification of alpha- and beta-tubulin, but also of other, yet unidentified protein substrates also detected by the antibody Gly-pep1. In immunofluorescence labeling, the antibody strongly labels glycylated microtubules. Source: Rabbit. Applications: ICC, IP, WB. Liquid. In PBS containing 0.02 % sodium azide. Microtubules are key cytoskeletal elements that are found in all eukaryotic cells. Microtubules fulfil a large range of different functions, which are thought to be controlled by the 'tubulin code' - mechanism to generate distinct microtubule identities. One mechanism to label specific microtubules are tubulin posttranslational modifications (PTMs), of which a large variety exists. One of these modifications is glycylation, which is generated by the addition of secondary (branched) glycine chains to the main (primary) peptide chain of the protein. The length of these branch chains can vary from one to more than 20 glycine residues. Glycylation is catalysed by the enzymes TTLL3, TTLL8 and TTLL10 from the tubulin tyrosine ligase-like (TTLL) family. Especially TTLL3 and TTLL8 are essential for the initiation of the glycylation because the generate the nascent glycine chain. The Gly-pep1 antibody was raised against a peptide mimicking beta2-tubulin (TUBB2A) with a single glycine branch on E437. The antibody specifically detects glycylated tubulin, and also other yet unknown glycylation substrates in cells as well as in tissues. As glycyation of microtubules is particularly found in cilia and flagella, Gly-pep1 labels motile cilia as well as primary cilia.
- 纯度
- >95 % (SDS-PAGE)
- 免疫原
- Synthetic peptide corresponding to D431EQGEFE(G-COOH*)EEEG441-NH2 of human Tubulin beta-2A chain (*secondary glycine branched from gamma-carboxyl group of glutamate as isopeptide bond).
- Top Product
- Discover our top product TUB Primary Antibody
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- Lot specific
- 缓冲液
- Liquid. In PBS containing 0.02 % sodium azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- After opening, prepare aliquots and store at -20 °C. Avoid freeze/thaw cycles.
- 储存条件
- 4 °C,-20 °C
- 储存方法
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Short Term Storage: +4°C
Long Term Storage: -20°C
Use & Stability: Stable for at least 1 year after receipt when stored at -20°C.
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- 抗原
- Tubulin (TUB)
- 别名
- Tubulin (TUB 产品)
- 别名
- 4t antibody, B4t antibody, BETA 98B antibody, CG4869 antibody, DTB1 antibody, Dmbeta4 antibody, Dmel\\CG4869 antibody, T antibody, Tub antibody, beta-Tub antibody, beta-Tub97EF antibody, beta-tub antibody, beta4 antibody, beta4t antibody, beta97F antibody, betaTub4 antibody, betaTub98BC antibody, betaTub98C antibody, beta-Tubulin at 97EF antibody, betaTub97EF antibody
- 背景
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Product Description: Microtubules are key cytoskeletal elements that are found in all eukaryotic cells. Microtubules fulfil a large range of different functions, which are thought to be controlled by the 'tubulin code' - mechanism to generate distinct microtubule identities. One mechanism to label specific microtubules are tubulin posttranslational modifications (PTMs), of which a large variety exists. One of these modifications is glycylation, which is generated by the addition of secondary (branched) glycine chains to the main (primary) peptide chain of the protein. The length of these branch chains can vary from one to more than 20 glycine residues. Glycylation is catalysed by the enzymes TTLL3, TTLL8 and TTLL10 from the tubulin tyrosine ligase-like (TTLL) family. Especially TTLL3 and TTLL8 are essential for the initiation of the glycylation because the generate the nascent glycine chain. The Gly-pep1 antibody was raised against a peptide mimicking beta2-tubulin (TUBB2A) with a single glycine branch on E437. The antibody specifically detects glycylated tubulin, and also other yet unknown glycylation substrates in cells as well as in tissues. As glycyation of microtubules is particularly found in cilia and flagella, Gly-pep1 labels motile cilia as well as primary cilia (Gadadhar et al. 2017)
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