Tubulin 抗体 (cleaved, EEVGVD Motif)
The 兔 多克隆 anti-Tubulin antibody (ABIN2444034) specifically detects Tubulin in WB 和 IHC.
The antibody is reactive with 人, 小鼠 和 Cow samples.
产品编号 ABIN2444034
发货至:
中国
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Quick Overview for Tubulin 抗体 (cleaved, EEVGVD Motif) (ABIN2444034)
抗原
See all Tubulin (TUB) 抗体
Tubulin (TUB)
适用
人, 小鼠, Cow
宿主
兔
克隆类型
多克隆
标记
This Tubulin antibody is un-conjugated
应用范围
Western Blotting (WB), Immunohistochemistry (IHC)
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抗原表位
- cleaved, EEVGVD Motif
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原理
- Alpha-Tubulin cleaved by caspase-6 Rabbit Polyclonal Antibody
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特异性
- The antibody recognizes the sequence (EEVGVD) in tubulin protein. It is specific for Caspase 6 cleaved α-Tubulin. The antibody was tested in IHC and western blots of samples with Alzheimer disease (AD) (Bovine brain sample, human samples, cells lines, mice hippocampus and whole brains). When tested in null mice with IHC and WB, no immunoreactivity was observed.
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免疫原
- EEVGVD peptide
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应用备注
- WB 1:1000,IHC 1:1000
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- If reconstituted with deionized water in 50 μL: WB 1:1000, IHC 1:1000. Optimal dilution has to be determined by the user.
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缓冲液
- Lyophilized serum
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储存液
- Without preservative
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储存条件
- 4 °C,-20 °C,-80 °C
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储存方法
- Lyophilized antibodies can be kept at 4°C for up to 3 months and should be kept at -20°C for long-term storage (2 years). To avoid freeze-thaw cycles, reconstituted antibodies should be aliquoted before freezing for long-term (1 year) storage (-80°C) or kept at 4°C for short-term usage (2 months). For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made with the assay buffer. After the maximum long-term storage period (2 years lyophilized or 1 year reconstituted) antibodies should be tested in your assay with a standard sample to verify if you have noticed any decrease in their efficacy. To limit antibody loss or degradation, BSA (final concentration 1%) and sodium azide (final concentration 0.02%) can be added to the suggested first dilution. It is important to first verify if those preservatives are compatible with your assay.
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有效期
- 24 months
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- Tubulin (TUB)
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别名
- Tubulin
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背景
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α-Tubulin cleaved by casp 6
Tubulin is the major constituent of microtubules. Microtubules are assembled from dimers of α- and β-tubulin. α-Tubulin cleaved by caspase-6 can be found in neurons, neurofibrillary tangles, neutrophil threads, and neuritic plaques in Alzheimer disease. α-Tubulin has been found to associate with the axonal microtubule-associated protein Tau, indicating that the cleavage of the functional α-Tubulin C-terminal domain by caspase-6 could alter the state of microtubules in neurons.
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UniProt
- Q71U36
抗原
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