Transferrin 抗体
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北京 101111
Quick Overview for Transferrin 抗体 (ABIN6252893)
抗原
See all Transferrin (TF) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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纯化方法
- PEG precipitation
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免疫原
- Neuraminidase-treated human red blood cells were used as the immunogen for the CD176 antibody.
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亚型
- IgM kappa
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anti-Transferrin (TF) (AA 361-683) antibody
TF 适用: 人 WB, IHC, ICC, IP 宿主: 兔 Polyclonal unconjugated
anti-Transferrin (TF) antibodyTF 适用: Cow WB, IHC, ICC, IP 宿主: 兔 Polyclonal unconjugated
anti-Transferrin (TF) antibodyTF 适用: Pig WB, IHC, ICC, IP 宿主: 兔 Polyclonal unconjugated
anti-Transferrin (TF) (AA 22-176) antibodyTF 适用: 小鼠 WB, IHC, ICC, IP 宿主: 兔 Polyclonal unconjugated
anti-Transferrin (TF) (AA 551-650) antibodyTF 适用: 人 WB, ELISA, IF 宿主: 小鼠 Monoclonal 1C2 unconjugated
anti-Transferrin (TF) antibodyTF 适用: 人 WB, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Transferrin (TF) antibodyTF 适用: 人 WB, IHC (p), IF (p) 宿主: 小鼠 Monoclonal 1F10 unconjugated
anti-Transferrin (TF) (AA 432-466) antibodyTF 适用: 人 WB, IHC (p), FACS 宿主: 兔 Polyclonal RB55980 unconjugated
anti-Transferrin (TF) (AA 101-135) antibodyTF 适用: 人 WB, IF 宿主: 兔 Polyclonal RB55977 unconjugated
anti-Transferrin (TF) antibodyTF 适用: 人 WB, IF, IHC (p) 宿主: 小鼠 Monoclonal 9B3 unconjugated
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应用备注
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Immunofluorescence: 0.5-1 μg/mL
Immunohistochemistry (FFPE): 3-5 μg/mL for 30 min at RT (1)
Optimal dilution of the CD176 antibody should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.2 mg/mL
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缓冲液
- PBS with 0.1 mg/mL BSA and 0.05 % sodium azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- Aliquot and Store at -20C. Avoid freez-thaw cycles.
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- Transferrin (TF)
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别名
- CD176
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背景
- Recognizes a disaccharide epitope, Gal1-3GalNAc, of Thomsen-Friedenreich (TF) antigen. It is specific for both anomeric forms of the disaccharide (TF and TF, including related structures on the glycolipid) and shows no cross-reactivity with sialylated glycophorin. The Thomsen-Friedenreich antigen acts as an oncofetal antigen, with low expression in normal adult tissues but increasing to fetal levels of expression in hyperplasia or malignancy. It is considered as a pan-carcinoma marker. During metastasis, the ability of malignant cells to form multicellular aggregates via homotypic or heterotypic aggregation and their adhesion to the endothelium are critical. The tumor-associated carbohydrate Thomsen-Friedenreich antigen (Gal-GalNAc) is involved in tumor cell adhesion and tissue invasion. It also causes an immune response, and overexpression of the antigen causes cancer cells to be more sensitive to natural killer cell lysis. The Thomsen-Friedenreich antigen is suppressed in normal healthy cells and represents one of the few chemically well-defined antigens associated with tumor malignancy. The presence of the Thomsen-Friedenreich antigen on the surface of cancer cells may result from a divergence from the normal pathway for O-linked glycosylation in these cells, most likely caused by inappropriate localization of the enzymes involved in synthesis of the disaccharide.
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途径
- Transition Metal Ion Homeostasis
抗原
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