pan Keratin 抗体
Quick Overview for pan Keratin 抗体 (ABIN6252867)
抗原
See all pan Keratin (panKRT) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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纯化方法
- Protein G affinity chromatography
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免疫原
- Recombinant human KRT77 and KRT76 protein were used as the immunogen for the pan Cytokeratin antibody.
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亚型
- IgG
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anti-pan Keratin (panKRT) antibody
panKRT 适用: 人, 大鼠, 小鼠 WB, IHC (p), IF (cc), IF (p) 宿主: 兔 Polyclonal unconjugated
anti-pan Keratin (panKRT) antibodypanKRT 适用: 人 IHC, ELISA, ICC 宿主: 小鼠 Monoclonal 7H8C4 unconjugated
anti-pan Keratin (panKRT) (AA 331-430) antibodypanKRT 适用: 人, 大鼠 IHC (p), IF (cc), IF (p), ICC, IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-pan Keratin (panKRT) (pan) antibodypanKRT 适用: 人, 大鼠, 小鼠 WB, IHC (p), ELISA 宿主: 兔 Polyclonal unconjugated
anti-pan Keratin (panKRT) antibodypanKRT 适用: 人 IHC 宿主: 兔 Monoclonal MSVA-000R unconjugated Recombinant Antibody
anti-pan Keratin (panKRT) antibodypanKRT 适用: 人, 大鼠, 小鼠, Cow, 猴, 犬, 兔, 小鸡 IF, FACS, WB, IHC (p) 宿主: 小鼠 Monoclonal AE1-AE3 unconjugated
anti-pan Keratin (panKRT) antibodyGMP Grade panKRT 适用: 人 FACS 宿主: 小鼠 Monoclonal MCK457 unconjugated
anti-pan Keratin (panKRT) antibody (iFluor™488)GMP Grade panKRT 适用: 人 FACS 宿主: 小鼠 Monoclonal MCK457 iFluor™488
anti-pan Keratin (panKRT) antibodypanKRT 适用: 人, 大鼠, 小鼠, Cow, Pig, Frog, 豚鼠, 山羊, Marmoset IF, FACS, WB, IHC, StM 宿主: 小鼠 Monoclonal SPM583 unconjugated
anti-pan Keratin (panKRT) antibodypanKRT 适用: 人, 大鼠 IF, FACS, IHC (p) 宿主: 小鼠 Monoclonal KRTL-1077-KRTH-1076 unconjugated
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应用备注
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Flow Cytometry: 0.5-1 μg/million cells in 0.1ml
Immunofluorescence: 1-2 μg/mL
Immunohistochemistry (FFPE): 0.25-0.5 μg/mL for 30 min at RT (1)
Prediluted format: incubate for 30 min at RT (2)
Optimal dilution of the pan Cytokeratin antibody should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min. -
限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.2 mg/mL
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缓冲液
- PBS with 0.1 mg/mL BSA and 0.05 % sodium azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- Aliquot and Store at -20C. Avoid freez-thaw cycles.
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- pan Keratin (panKRT)
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别名
- pan Cytokeratin
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背景
- Twenty human keratins are resolved with two-dimensional gel electrophoresis into acidic (pI 6.0) subfamilies. This antibody cocktail recognizes acidic (Type I or LMW) and basic (Type II or HMW) cytokeratins, which include Keratins 1, 3, 4, 5, 6, 8, 10, 14, 15, 16, and 19. Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis. KRTL/KRTH is a broad spectrum anti pan-cytokeratin antibody cocktail, which differentiates epithelial tumors from non-epithelial tumors e.g. squamous vs. adenocarcinoma of the lung, liver carcinoma, breast cancer, and esophageal cancer. It has been used to characterize the source of various neoplasms and to study the distribution of cytokeratin containing cells in epithelia during normal development and during the development of epithelial neoplasms. This antibody stains cytokeratins present in normal and abnormal human tissues and has shown high sensitivity in the recognition of epithelial cells and carcinomas.
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基因ID
- 43769
抗原
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