GFM1 抗体
Quick Overview for GFM1 抗体 (ABIN6252740)
抗原
See all GFM1 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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特异性
- Does not react with mouse and rat
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纯化方法
- Protein G affinity chromatography
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免疫原
- A semi-purified mitochondrial preparation was used as the immunogen for the anti-Mitochondrial antibody.
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亚型
- IgG1 kappa
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anti-G-Elongation Factor, Mitochondrial 1 (GFM1) (AA 511-751) antibody
GFM1 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-G-Elongation Factor, Mitochondrial 1 (GFM1) antibodyGFM1 适用: 人, 小鼠, 大鼠 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-G-Elongation Factor, Mitochondrial 1 (GFM1) (AA 482-751) antibodyGFM1 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-G-Elongation Factor, Mitochondrial 1 (GFM1) (AA 1-158) antibodyGFM1 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-G-Elongation Factor, Mitochondrial 1 (GFM1) (C-Term) antibodyGFM1 适用: 人 WB, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-G-Elongation Factor, Mitochondrial 1 (GFM1) antibodyGFM1 适用: 人 WB, IF, IHC (p), FACS 宿主: 小鼠 Monoclonal MTC719 unconjugated
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应用备注
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ELISA: order Ab without BSA
Immunofluorescence: 0.5-1 μg/mL
Western blot: 0.25-0.5 μg/mL
Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT (1)
Prediluted format: incubate for 30 min at RT (2)
Optimal dilution of the anti-Mitochondrial antibody should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 min.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min. -
限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.2 mg/mL
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缓冲液
- PBS with 0.1 mg/mL BSA and 0.05 % sodium azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- Aliquot and Store at -20C. Avoid freez-thaw cycles.
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- GFM1 (G-Elongation Factor, Mitochondrial 1 (GFM1))
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别名
- Mitochondrial
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背景
- This mAb recognizes a 60 kDa antigen associated with the mitochondria in human cells. It is a part of a new panel of reagents, which recognizes subcellular organelles or compartments of human cells. These markers may be useful in identification of these organelles in cells, tissues, and biochemical preparations. It recognizes an antigen associated with the mitochondria in human cells only. It can be used to stain the mitochondria in cell or tissue preparations and can be used as a mitochondrial marker in subcellular fractions. It produces a spaghetti-like pattern in normal and malignant cells and may be used to stain mitochondria of cells in fixed or frozen tissue sections. It can also be used with paraformaldehyde fixed frozen tissue or cell preparations. This mAb is an excellent marker for human cells in xenographic model research. It reacts specifically with human cells, including neurons and embryonic stem cells.
抗原
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