TDP2 抗体
Quick Overview for TDP2 抗体 (ABIN6252692)
抗原
See all TDP2 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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纯化方法
- Protein G affinity chromatography
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免疫原
- Human recombinant full length protein was used as the immunogen for this TDP2 antibody.
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亚型
- IgG2b kappa
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anti-tyrosyl-DNA phosphodiesterase 2 (TDP2) (AA 1-362) antibody
TDP2 适用: 人 WB, ELISA, IHC (p) 宿主: 小鼠 Monoclonal 3D11 unconjugated
anti-tyrosyl-DNA phosphodiesterase 2 (TDP2) (AA 1-362) antibodyTDP2 适用: 人 WB, IF 宿主: 小鼠 Polyclonal unconjugated
anti-tyrosyl-DNA phosphodiesterase 2 (TDP2) (AA 246-272), (C-Term) antibodyTDP2 适用: 人 WB, IF, FACS, IHC (p) 宿主: 兔 Polyclonal RB20005 unconjugated
anti-tyrosyl-DNA phosphodiesterase 2 (TDP2) (Middle Region) antibodyTDP2 适用: 人, 小鼠, 大鼠, 兔, Cow, 犬, 马, 豚鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-tyrosyl-DNA phosphodiesterase 2 (TDP2) (AA 253-281), (C-Term) antibodyTDP2 适用: 人 WB, IF, FACS, IHC (p), EIA 宿主: 兔 Polyclonal unconjugated
anti-tyrosyl-DNA phosphodiesterase 2 (TDP2) antibodyTDP2 适用: 人 IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-tyrosyl-DNA phosphodiesterase 2 (TDP2) (AA 154-203) antibodyTDP2 适用: 人, 小鼠, 大鼠, 兔, 豚鼠, Bat, 猴, 斑马鱼 WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-tyrosyl-DNA phosphodiesterase 2 (TDP2) antibodyGMP Grade TDP2 适用: 人 WB, FACS 宿主: 小鼠 Monoclonal 42c unconjugated
anti-tyrosyl-DNA phosphodiesterase 2 (TDP2) (AA 29-305) antibodyTDP2 适用: 人 WB, IHC, IF, IHC (p), ICC 宿主: 兔 Polyclonal unconjugated
anti-tyrosyl-DNA phosphodiesterase 2 (TDP2) antibodyTDP2 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal unconjugated
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应用备注
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Flow Cytometry: 0.5-1 μg/10^6 cells in 0.1ml
IF: 0.5-1 μg/mL
IHC (FFPE): 0.5-1 μg/mL for 30 min at RT
The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the TDP2 antibody to be titered up or down for optimal performance. -
限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.2 mg/mL
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缓冲液
- PBS with 0.1 mg/mL BSA and 0.05 % sodium azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- Aliquot and Store at -20C. Avoid freez-thaw cycles.
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- TDP2 (tyrosyl-DNA phosphodiesterase 2 (TDP2))
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别名
- TDP2
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背景
- This mAb recognizes a protein of 41 kDa, which is identified as TDP2, or ETS1 associated protein II. It is a member of a superfamily of divalent cation-dependent phosphodiesterases. The encoded protein associates with CD40, tumor necrosis factor (TNF) receptor-75 and TNF receptor associated factors (TRAFs), and inhibits nuclear factor-kappa-B activation. This protein has sequence and structural similarities with APE1 endonuclease, which is involved in both DNA repair and the activation of transcription factors. DNA repair enzyme that can remove a variety of covalent adducts from DNA through hydrolysis of a 5'-phosphodiester bond, giving rise to DNA with a free 5' phosphate. Catalyzes the hydrolysis of dead-end complexes between DNA and the topoisomerase 2 (TOP2) active site tyrosine residue. Hydrolyzes 5'-phosphoglycolates on protruding 5' ends on DNA double-strand breaks (DSBs) due to DNA damage by radiation and free radicals. The 5'-tyrosyl DNA phosphodiesterase activity can enable the repair of TOP2-induced DSBs without the need for nuclease activity, creating a 'clean' DSB with 5'-phosphate termini that are ready for ligation. Has also 3'-tyrosyl DNA phosphodiesterase activity, but less efficiently and much slower than TDP1. May also act as a negative regulator of ETS1 and may inhibit nuclear factor-kappa-B activation.
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基因ID
- 51567
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UniProt
- O95551
抗原
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