ECM1 抗体
Quick Overview for ECM1 抗体 (ABIN6252532)
抗原
See all ECM1 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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纯化方法
- Protein G affinity chromatography
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免疫原
- Secretory Component protein isolated from human colostrum was used as the immunogen for this Secretory Component antibody.
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亚型
- IgG1 kappa
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anti-Extracellular Matrix Protein 1 (ECM1) (AA 488-567) antibody
ECM1 适用: 人, 小鼠 WB, ELISA, FACS, IHC (p), IHC (fro), IF (p), IF (cc) 宿主: 兔 Polyclonal unconjugated
anti-Extracellular Matrix Protein 1 (ECM1) (AA 20-434) antibodyECM1 适用: 小鼠 IHC, WB, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Extracellular Matrix Protein 1 (ECM1) (AA 150-250) antibodyECM1 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-Extracellular Matrix Protein 1 (ECM1) (AA 386-540) antibodyECM1 适用: 人 IHC, WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Extracellular Matrix Protein 1 (ECM1) antibodyECM1 适用: 人, 大鼠 IHC, IF, FACS, StM 宿主: 小鼠 Monoclonal SC05 unconjugated
anti-Extracellular Matrix Protein 1 (ECM1) antibodyECM1 适用: 人, 大鼠 IHC, StM 宿主: 兔 Monoclonal ECM1-2889R unconjugated Recombinant Antibody
anti-Extracellular Matrix Protein 1 (ECM1) antibodyECM1 适用: 人, 大鼠 IHC, StM 宿主: 小鼠 Monoclonal rECM1-792 unconjugated Recombinant Antibody
anti-Extracellular Matrix Protein 1 (ECM1) (C-Term) antibodyECM1 适用: 人 WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Extracellular Matrix Protein 1 (ECM1) (Middle Region) antibodyECM1 适用: 人, 大鼠, 小鼠, Cow, 犬, 豚鼠, 马, Pig, 兔 IHC, WB 宿主: 兔 Polyclonal unconjugated
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应用备注
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Flow Cytometry: 0.5-1 μg/million cells in 0.1ml
Immunofluorescence: 1-2 μg/mL
Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 minutes at RT (1)
Prediluted format: incubate for 30 min at RT (2)
The optimal dilution of the Secretory Component antibody for each application should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min. -
限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.2 mg/mL
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缓冲液
- PBS with 0.1 mg/mL BSA and 0.05 % sodium azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- Aliquot and Store at -20C. Avoid freez-thaw cycles.
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- ECM1 (Extracellular Matrix Protein 1 (ECM1))
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别名
- Extracellular Matrix Protein 1
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背景
- This mAb reacts with a reduction-resistant epitope present in both free and SIgA bound Secretory Component. It does not react with the cell lines lacking secretory component. The antibody is useful for studying the distribution and level of both free and bound secretory component. Secretory component is differentially expressed in epithelium, and the antibody is a popular marker for identifying subpopulations of epithelial cells and epithelial differentiation. The Secretory component antibody is a useful research tool for studying mucosal immunity, inflammation, remodeling, differentiation and tumorigenesis, all processes associated with differential secretory component expression.
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基因ID
- 1893
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UniProt
- Q16610
抗原
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