Calponin 抗体
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北京 101111
Quick Overview for Calponin 抗体 (ABIN6252459)
抗原
适用
宿主
克隆类型
标记
应用范围
克隆位点
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纯化方法
- Protein G affinity chromatography
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免疫原
- Crude human uterus extract was used as the immunogen for the Calponin antibody.
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亚型
- IgG1 kappa
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anti-Calponin (AA 1-298) antibody
适用: 人 IHC (p), WB, IHC, ELISA 宿主: 小鼠 Monoclonal 2F5-1H4 unconjugated
anti-Calponin antibody适用: 人, 大鼠 IHC (p), IF, FACS 宿主: 小鼠 Monoclonal CNN1-832-CALP unconjugated
anti-Calponin (N-Term) antibody适用: 人, 大鼠, 小鼠, 犬, 猴, Cow, 马, 绵羊 WB 宿主: 兔 Polyclonal unconjugated
anti-Calponin antibody适用: 人, 大鼠 IHC (p), IF, FACS 宿主: 兔 Monoclonal CNN1-1408R unconjugated Recombinant Antibody
anti-Calponin antibody适用: 人, 大鼠 IHC (p), IF, FACS 宿主: 小鼠 Monoclonal CNN1-832 unconjugated
anti-Calponin antibody适用: 人, 大鼠 IHC (p), IF, FACS 宿主: 小鼠 Monoclonal CNN1-832-CALP unconjugated
anti-Calponin antibody适用: 人 IHC (p), IF, FACS 宿主: 兔 Monoclonal RMCN1-1 unconjugated Recombinant Antibody
anti-Calponin antibody适用: 人, 大鼠 IHC (p), IF, FACS 宿主: 兔 Monoclonal CNN1-1408R unconjugated Recombinant Antibody
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应用备注
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Flow Cytometry: 0.5-1 μg/million cells in 0.1ml
Immunofluorescence: 1-2 μg/mL
Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT (1)
Optimal dilution of the Calponin antibody should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 min
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.2 mg/mL
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缓冲液
- PBS with 0.1 mg/mL BSA and 0.05 % sodium azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- Aliquot and Store at -20C. Avoid freez-thaw cycles.
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- Calponin
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背景
- Multiple isoelectric variants of calponin have been identified, however only two molecular weight isoforms exist, a 34 kDa form and a 29 kDa form. Expression of the 29 kDa form, I-calponin, is primarily restricted to muscle of the urogenital tract, whereas the higher molecular weight variant has been demonstrated in vascular and visceral smooth muscle. In Western blotting, this mAb reacts with only the 34 kDa form of calponin in extracts of human aortic medial smooth muscle and is unreactive with fibroblast extracts of cultivated human foreskin. Calponin is a calmodulin, F-actin and tropomyosin binding protein, which is thought to be involved in the regulation of smooth muscle contraction. Calponin expression is restricted to smooth muscle cells and has been shown to be a marker of the differentiated (contractile) phenotype of developing smooth muscle.
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基因ID
- 1264
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UniProt
- P51911
抗原
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