MCM7 抗体 (AA 195-319)
Quick Overview for MCM7 抗体 (AA 195-319) (ABIN6252451)
抗原
See all MCM7 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- AA 195-319
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纯化方法
- Protein G affinity chromatography
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免疫原
- A human partial recombinant protein corresponding to amino acids 195-319 was used as the immunogen for the MCM7 antibody.
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亚型
- IgG2a kappa
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应用备注
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Immunofluorescence: 0.5-1 μg/mL
Flow Cytometry: 0.5-1 μg/million cells in 0.1ml
Immunohistochemistry (FFPE): 1-2 μg/mL for 30 min at RT
Prediluted IHC only format: incubate for 30 min at RT (1)
Titering of the MCM7 antibody may be required for optimal performance.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.2 mg/mL
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缓冲液
- PBS with 0.1 mg/mL BSA and 0.05 % sodium azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- Aliquot and Store at -20C. Avoid freez-thaw cycles.
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- MCM7 (Minichromosome Maintenance Complex Component 7 (MCM7))
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别名
- MCM7
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背景
- MCM7 is one of the highly conserved mini-chromosome maintenance proteins (MCM) that is essential for the initiation of eukaryotic genome replication. The hexameric protein complex formed by the MCM proteins is a key component of the pre-replication complex and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. The MCM complex consisting of this protein and MCM2, 4 and 6 proteins possesses DNA helicase activity, and may act as a DNA unwinding enzyme. Cyclin D1-dependent kinase, CDK4, is found to associate with this protein, and may regulate the binding of this protein with the tumor suppressor protein RB1/RB.
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基因ID
- 4176
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UniProt
- P33993
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途径
- DNA Damage Repair, Mitotic G1-G1/S Phases, DNA Replication, Chromatin Binding, Synthesis of DNA
抗原
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