CD45RO 抗体
Our Local Distributor
北京 101111
Quick Overview for CD45RO 抗体 (ABIN6252434)
抗原
适用
宿主
克隆类型
标记
应用范围
克隆位点
-
-
纯化方法
- Protein G affinity chromatography
-
免疫原
- Cultured human T-cells from an IL-2-dependent T-cell line (CA1) were used as immunogen for this anti-CD45RO antibody.
-
亚型
- IgG2a kappa
-
-
anti-CD45RO (CD45RO) antibody
CD45RO 适用: 人 FACS, IHC, WB, IF (p), ICC, mIF 宿主: 小鼠 Monoclonal UCHL-1 unconjugated
anti-CD45RO (CD45RO) antibodyCD45RO 适用: 人 WB, IHC (p), IF (p) 宿主: 兔 Polyclonal unconjugated
anti-CD45RO (CD45RO) antibody (PE)CD45RO 适用: Cow, 山羊 FACS 宿主: 小鼠 Monoclonal IL-A116 PE
anti-CD45RO (CD45RO) antibodyGMP Grade CD45RO 适用: 人 FACS, IHC, IP 宿主: 小鼠 Monoclonal UCHL1 unconjugated
anti-CD45RO (CD45RO) antibody (PE)GMP Grade (Analyte) CD45RO 适用: 人 FACS 宿主: 小鼠 Monoclonal UCHL1 PE
anti-CD45RO (CD45RO) antibody (PE-CF®594)GMP Grade (Analyte) CD45RO 适用: 人 FACS 宿主: 小鼠 Monoclonal UCHL1 PE-CF®594
anti-CD45RO (CD45RO) antibody (FITC)GMP Grade CD45RO 适用: 人 FACS 宿主: 小鼠 Monoclonal UCHL1 FITC
anti-CD45RO (CD45RO) antibody (mFluor™450)GMP Grade CD45RO 适用: 人 FACS 宿主: 小鼠 Monoclonal UCHL1 mFluor™450
anti-CD45RO (CD45RO) antibody (APC)GMP Grade CD45RO 适用: 人 FACS 宿主: 小鼠 Monoclonal UCHL1 APC
-
-
应用备注
-
Flow Cytometry: 0.5-1 μg/million cells
Immunofluorescence: 0.5-1 μg/mL
Immunohistology (FFPE): 0.25-0.5 μg/mL for 30 minutes at RT (1)
Prediluted format : incubate for 30 min at RT (2)
Due to variation in protocol and secondary antibody used, the anti-CD45RO antibody may need to be titered for optimal performance.1. FFPE staining requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min. -
限制
- 仅限研究用
-
-
-
状态
- Liquid
-
浓度
- 0.2 mg/mL
-
缓冲液
- PBS with 0.1 mg/mL BSA and 0.05 % sodium azide
-
储存液
- Sodium azide
-
注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
储存条件
- -20 °C
-
储存方法
- Aliquot and Store at -20C. Avoid freez-thaw cycles.
-
-
- CD45RO
-
别名
- CD45RO
-
背景
- Recognizes a 180-185 kDa protein, identified as isoform of leukocyte common antigen (CD45RO). This antibody reacts with mature activated T-cells, most thymocytes, and a sub-population of resting T-cells within both CD4 and CD8 subsets. It shows no reactivity with normal B or natural killer cells, but reacts with granulocytes and monocytes. Reportedly, it is useful to identify T-cell lymphomas and leukemias. It rarely stains NK cells or B-cell lymphomas.
-
基因ID
- 5788
-
UniProt
- P08575
抗原
-
