kappa Light Chain 抗体
Quick Overview for kappa Light Chain 抗体 (ABIN6252341)
抗原
See all kappa Light Chain 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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特异性
- Does not react with rat
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纯化方法
- Protein G affinity chromatography
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免疫原
- Human B-Lymphoma Cells were used as the immunogen for this Kappa light chain antibody.
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亚型
- IgG1 kappa
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anti-kappa Light Chain antibody (FITC)
适用: 人 FACS 宿主: 小鼠 Monoclonal A8B5 FITC
anti-kappa Light Chain antibody (PE)适用: 人 FACS 宿主: 小鼠 Monoclonal TB28-2 PE
anti-kappa Light Chain antibody适用: 人 FACS, IHC (p), ELISA, IHC (fro) 宿主: 小鼠 Monoclonal A8B5 unconjugated
anti-kappa Light Chain antibody适用: 人 FACS, WB 宿主: 小鼠 Monoclonal TB28-2 unconjugated
anti-kappa Light Chain antibody适用: 人 FACS, WB, IHC (p), ELISA, IP, ICC 宿主: 小鼠 Monoclonal MEM-09 unconjugated
anti-kappa Light Chain antibody (PE)GMP Grade 适用: 人 FACS 宿主: 小鼠 Monoclonal TB 28-2 PE
anti-kappa Light Chain antibody (Biotin)适用: 人 FACS 宿主: 小鼠 Monoclonal TB28-2 Biotin
anti-kappa Light Chain (AA 47-63), (N-Term) antibody适用: 人 IHC (p) 宿主: 兔 Monoclonal A21-Y unconjugated
anti-kappa Light Chain antibody (APC)适用: 人 FACS 宿主: 小鼠 Monoclonal TB28-2 APC
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应用备注
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Flow Cytometry: 0.5-2.0 μg/million cells
IF: 1-2 μg/mL
WB: 0.5-1 μg/mL
IHC (FFPE): 0.5-1 μg/mL for 30 min at RT (1)
Prediluted format : incubate for 30 min at RT (2)
The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the antibody to be titered up or down for optimal performance.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min. -
限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.2 mg/mL
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缓冲液
- PBS with 0.1 mg/mL BSA and 0.05 % sodium azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- Aliquot and Store at -20C. Avoid freez-thaw cycles.
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- kappa Light Chain
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背景
- This antibody is specific to the kappa light chain of immunoglobulin and shows no cross-reaction with lambda light chain or any of the five heavy chains. In mammals, the two light chains in an antibody are always identical, with only one type of light chain, kappa or lambda. In general, the ratio of Kappa to Lambda is 3:1. However, with the occurrence of multiple myeloma or other B-cell malignancies this ratio is disturbed. Antibody to the kappa light chain is reportedly useful in the identification of leukemias, plasmacytomas, and certain non-Hodgkin's lymphomas. Demonstration of clonality in lymphoid infiltrates indicates that the infiltrate is malignant.
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基因ID
- 3514
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UniProt
- P01834, P01601
抗原
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