MLANA 抗体
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- 抗原 See all MLANA 抗体
- MLANA (Melan A (MLANA))
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适用
- 人, 小鼠, 大鼠
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This MLANA antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (IHC), ELISA, Immunoprecipitation (IP)
- 纯化方法
- Protein G affinity chromatography
- 免疫原
- Recombinant human Melan-A protein was used as the immunogen for this antibody.
- 克隆位点
- M2-9E3
- 亚型
- IgG2b kappa
- Top Product
- Discover our top product MLANA Primary Antibody
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anti-Melan A (MLANA) antibody
MLANA 适用: 人, 小鼠, 大鼠 IHC, ISt, StM 宿主: 兔 Monoclonal MLANA-1761R unconjugated Recombinant Antibody
anti-Melan A (MLANA) antibodyMLANA 适用: 人, 小鼠, 大鼠 WB, IF, FACS, IHC, ISt, StM 宿主: 小鼠 Monoclonal M2-9E3 unconjugated
anti-Melan A (MLANA) antibodyMLANA 适用: 人, 马 IHC, ISt, StM 宿主: 小鼠 Monoclonal MLANA-1761R unconjugated
anti-Melan A (MLANA) (AA 48-118) antibodyMLANA 适用: 人 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Melan A (MLANA) (AA 1-118) antibodyMLANA 适用: 人 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Melan A (MLANA) antibodyMLANA 适用: 人, 小鼠, 大鼠, 犬 IHC, ISt, StM 宿主: 小鼠 Monoclonal rMLANA-788 unconjugated Recombinant Antibody
anti-Melan A (MLANA) antibodyMLANA 适用: 人, 小鼠, 大鼠, 犬 IHC, ISt, StM 宿主: 兔 Monoclonal MLANA-1409R unconjugated Recombinant Antibody
anti-Melan A (MLANA) antibodyMLANA 适用: 人, 小鼠, 大鼠, 犬 WB, IF, FACS, IHC, ISt, StM 宿主: 小鼠 Monoclonal A103 unconjugated
anti-Melan A (MLANA) antibodyMLANA 适用: 人 IHC 宿主: 小鼠 Monoclonal MSVA-901M- unconjugated Recombinant Antibody
anti-Melan A (MLANA) (AA 48-118) antibodyMLANA 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 2F3B7 unconjugated
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- 应用备注
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ELISA: order BSA-free format for coating
Flow Cytometry: 0.5-1 μg/million cells
IF: 1-2 μg/mL
WB: 0.5-1 μg/mL
IP: 0.5-1 μg/500 μg protein lysate
IHC (FFPE): 0.5-1 μg/mL for 30 minutes at RT (1)
Prediluted format : incubate for 30 min at RT (2)
The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the Melan-A antibody to be titered up or down for optimal performance.1. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min. - 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 0.2 mg/mL
- 缓冲液
- PBS with 0.1 mg/mL BSA and 0.05 % sodium azide
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- -20 °C
- 储存方法
- Aliquot and Store at -20C. Avoid freez-thaw cycles.
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- 抗原
- MLANA (Melan A (MLANA))
- 别名
- Melan-A (MLANA 产品)
- 别名
- A930034P04Rik antibody, Mart1 antibody, MART-1 antibody, MART1 antibody, melan-A antibody, Mlana antibody, MLANA antibody
- 背景
- This antibody recognizes a protein doublet of 20-22 kDa, identified as MART-1 (Melanoma Antigen Recognized by T cells 1) or Melan-A. MART-1 is a melanocyte differentiation antigen recognized by autologous cytotoxic T lymphocytes. There are seven other melanoma associated antigens recognized by autologous cytotoxic T cells: MAGE-1, MAGE-3, tyrosinase, gp100, gp75, BAGE-1, and GAGE-1. Subcellular fractionation shows that MART-1 is present in melanosomes and endoplasmic reticulum. This MART-1 antibody labels melanomas and other tumors showing melanocytic differentiation. It is also a useful positive-marker for angiomyolipomas. The antibody does not stain tumor cells of epithelial, lymphoid, glial, or mesenchymal origin.
- 基因ID
- 2315
- UniProt
- Q16655
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