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KRT14 抗体 (C-Term)

This anti-KRT14 antibody is a 小鼠 单克隆 antibody detecting KRT14 in WB, IHC, IF 和 FACS. Suitable for 人, 大鼠 和 小鼠.
产品编号 ABIN6252305
发货至: 中国

Quick Overview for KRT14 抗体 (C-Term) (ABIN6252305)

抗原

See all KRT14 抗体
KRT14 (Keratin 14 (KRT14))

适用

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人, 大鼠, 小鼠

宿主

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小鼠

克隆类型

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  • 1
单克隆

标记

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This KRT14 antibody is un-conjugated

应用范围

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Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

克隆位点

LL002
  • 抗原表位

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    C-Term

    纯化方法

    Protein G affinity chromatography

    免疫原

    A synthetic peptide of 15 amino acid from the C-terminus of human Cytokeratin 14 was used as the immunogen.

    亚型

    IgG3
  • 应用备注

    Western blot: 0.5-1 μg/mL

    Flow Cytometry: 0.5-1 μg/million cells

    IF: 1-2 μg/mL

    IHC (FFPE): 1-2 μg/mL for 30 min at RT (1)

    Prediluted format : incubate for 30 min at RT (2)

    The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the antibody to be titered up or down for optimal performance.

    1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM Citrate Buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
    2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    0.2 mg/mL

    缓冲液

    PBS with 0.1 mg/mL BSA and 0.05 % sodium azide

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    储存条件

    -20 °C

    储存方法

    Aliquot and Store at -20C. Avoid freez-thaw cycles.
  • 抗原

    KRT14 (Keratin 14 (KRT14))

    别名

    Cytokeratin 14

    背景

    Cytokeratin 14 (CK14) belongs to the type I (or A or acidic) subfamily of low molecular weight keratins and exists in combination with keratin 5 (type II or B or basic). Cytokeratin 14 is found in basal cells of squamous epithelia, some glandular epithelia, myoepithelium, and mesothelial cells. antibody to cytokeratin 14 is useful in differentiating squamous cell carcinomas from poorly differentiated epithelial tumors. cytokeratin 14 antibody is one of the specific basal markers for distinguishing between basal and non-basal subtypes of breast carcinomas. Cytokeratin 14 antibody is also a good marker for differentiation of intraductal from invasive salivary duct carcinoma by the positive staining of basal cells surrounding the in-situ neoplasm as well as for differentiation of benign prostate from prostate carcinoma. Furthermore, this antibody has been useful in separating oncocytic tumors of the kidney from its renal mimics, and in identifying metaplastic carcinomas of the breast.

    基因ID

    3861

    UniProt

    P02533
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