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VEGF 抗体

This 小鼠 单克隆 anti-VEGF antibody (Clone VEGF-1063) specifically detects VEGF in WB, IF 和 IHC (p). The antibody is reactive with 人, 小鼠, 大鼠, 犬 和 兔 samples.
产品编号 ABIN6252287
发货至: 中国
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中国
北京 101111
No. 88 KeChuang 6th Street
Beijing Economic Technological Development Area
Room 801-803
4A Biotech Co.,Ltd.
Tel +86 (0512) 65829739 传真 +86 (010) 6788 5057

Quick Overview for VEGF 抗体 (ABIN6252287)

抗原

See all VEGF 抗体
VEGF (Vascular Endothelial Growth Factor (VEGF))

适用

  • 88
  • 47
  • 19
  • 4
  • 3
  • 2
  • 2
  • 1
  • 1
人, 小鼠, 大鼠, 犬, 兔

宿主

  • 50
  • 45
  • 13
  • 3
  • 2
  • 2
  • 2
小鼠

克隆类型

  • 66
  • 48
  • 1
单克隆

标记

  • 52
  • 17
  • 5
  • 5
  • 4
  • 4
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
This VEGF antibody is un-conjugated

应用范围

  • 76
  • 61
  • 51
  • 23
  • 20
  • 20
  • 15
  • 15
  • 12
  • 9
  • 6
  • 4
  • 3
  • 3
  • 3
  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

克隆位点

VEGF-1063
  • 纯化方法

    Protein G affinity chromatography

    免疫原

    Human recombinant protein was used as the immunogen for the VEGF antibody.

    亚型

    IgG1 kappa
  • 应用备注

    Immunofluorescence: 1-2 μg/mL

    Immunohistochemistry (FFPE): 2-4 μg/mL for 30 min at RT (1)

    Optimal dilution of the VEGF antibody should be determined by the researcher.

    1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM Tris with 1  mM EDTA,  pH 9.0, for 10-20 min followed by cooling at RT for 20 min.

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    0.2 mg/mL

    缓冲液

    PBS with 0.1 mg/mL BSA and 0.05 % sodium azide

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    储存条件

    -20 °C

    储存方法

    Aliquot and Store at -20C. Avoid freez-thaw cycles.
  • 抗原

    VEGF (Vascular Endothelial Growth Factor (VEGF))

    别名

    VEGF

    背景

    This mAb recognizes proteins of 19-22  kDa (reducing) and 38  kDa-44  kDa (non-reducing), identified as various isoforms of Vascular Endothelial Growth Factor or Vascular Permeability Factor (VEGF/VPF). It is highly specific to VEGF, which is a homodimeric, disulfide-linked glycoprotein with a close homology to platelet derived growth factor (PDGF). There are multiple isoforms of VEGF containing 206-, 189-, 165-, and 121-amino acid residues. The smaller two isoforms, VEGF165 and VEGF121, are secreted proteins and act as diffusible agents, whereas the larger two remain cell associated. VEGF/VPF plays an important role in angiogenesis, which promotes tumor progression and metastasis.

    基因ID

    7422

    UniProt

    P15692
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