VEGF 抗体
Quick Overview for VEGF 抗体 (ABIN6252287)
抗原
See all VEGF 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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纯化方法
- Protein G affinity chromatography
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免疫原
- Human recombinant protein was used as the immunogen for the VEGF antibody.
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亚型
- IgG1 kappa
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应用备注
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Immunofluorescence: 1-2 μg/mL
Immunohistochemistry (FFPE): 2-4 μg/mL for 30 min at RT (1)
Optimal dilution of the VEGF antibody should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 min.
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.2 mg/mL
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缓冲液
- PBS with 0.1 mg/mL BSA and 0.05 % sodium azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- Aliquot and Store at -20C. Avoid freez-thaw cycles.
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- VEGF (Vascular Endothelial Growth Factor (VEGF))
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别名
- VEGF
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背景
- This mAb recognizes proteins of 19-22 kDa (reducing) and 38 kDa-44 kDa (non-reducing), identified as various isoforms of Vascular Endothelial Growth Factor or Vascular Permeability Factor (VEGF/VPF). It is highly specific to VEGF, which is a homodimeric, disulfide-linked glycoprotein with a close homology to platelet derived growth factor (PDGF). There are multiple isoforms of VEGF containing 206-, 189-, 165-, and 121-amino acid residues. The smaller two isoforms, VEGF165 and VEGF121, are secreted proteins and act as diffusible agents, whereas the larger two remain cell associated. VEGF/VPF plays an important role in angiogenesis, which promotes tumor progression and metastasis.
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基因ID
- 7422
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UniProt
- P15692
抗原
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