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VEGF 抗体

This anti-VEGF antibody is a 小鼠 单克隆 antibody detecting VEGF in WB, IHC (p) 和 IF. Suitable for 人, 小鼠, 大鼠, 犬 和 兔.
产品编号 ABIN6252287
发货至: 中国

Quick Overview for VEGF 抗体 (ABIN6252287)

抗原

See all VEGF 抗体
VEGF (Vascular Endothelial Growth Factor (VEGF))

适用

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人, 小鼠, 大鼠, 犬, 兔

宿主

  • 43
  • 40
  • 14
  • 2
  • 2
  • 1
小鼠

克隆类型

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单克隆

标记

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This VEGF antibody is un-conjugated

应用范围

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Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF)

克隆位点

VEGF-1063
  • 纯化方法

    Protein G affinity chromatography

    免疫原

    Human recombinant protein was used as the immunogen for the VEGF antibody.

    亚型

    IgG1 kappa
  • 应用备注

    Immunofluorescence: 1-2 μg/mL

    Immunohistochemistry (FFPE): 2-4 μg/mL for 30 min at RT (1)

    Optimal dilution of the VEGF antibody should be determined by the researcher.

    1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM Tris with 1  mM EDTA,  pH 9.0, for 10-20 min followed by cooling at RT for 20 min.

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    0.2 mg/mL

    缓冲液

    PBS with 0.1 mg/mL BSA and 0.05 % sodium azide

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    储存条件

    -20 °C

    储存方法

    Aliquot and Store at -20C. Avoid freez-thaw cycles.
  • 抗原

    VEGF (Vascular Endothelial Growth Factor (VEGF))

    别名

    VEGF

    背景

    This mAb recognizes proteins of 19-22  kDa (reducing) and 38  kDa-44  kDa (non-reducing), identified as various isoforms of Vascular Endothelial Growth Factor or Vascular Permeability Factor (VEGF/VPF). It is highly specific to VEGF, which is a homodimeric, disulfide-linked glycoprotein with a close homology to platelet derived growth factor (PDGF). There are multiple isoforms of VEGF containing 206-, 189-, 165-, and 121-amino acid residues. The smaller two isoforms, VEGF165 and VEGF121, are secreted proteins and act as diffusible agents, whereas the larger two remain cell associated. VEGF/VPF plays an important role in angiogenesis, which promotes tumor progression and metastasis.

    基因ID

    7422

    UniProt

    P15692
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