Melanoma 抗体
Our Local Distributor
北京 101111
Quick Overview for Melanoma 抗体 (ABIN6252230)
抗原
适用
宿主
克隆类型
标记
应用范围
克隆位点
-
-
特异性
- Does not react with rat
-
纯化方法
- Protein G affinity chromatography
-
免疫原
- Membranes from a human melanoma metastasis were used as the immunogen for this Melanoma antibody (gp100).
-
亚型
- IgG2b kappa
-
-
anti-Melanoma antibody
适用: 人, 马 IHC (p), IF, FACS 宿主: 小鼠 Monoclonal NKI-beteb unconjugated
anti-Melanoma antibody适用: 人, 犬, 小鼠 IHC (p), IF 宿主: 小鼠 Monoclonal PNL2 unconjugated
-
-
应用备注
-
Flow Cytometry: 0.5-1 μg/million cells
IF: 1-2 μg/mL
IHC (FFPE): 1-2 μg/mL for 30 minutes at RT (1)
Differences in protocols and secondaries may require the gp100 antibody to be titered up or down for optimal performance.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
-
限制
- 仅限研究用
-
-
-
状态
- Liquid
-
浓度
- 0.2 mg/mL
-
缓冲液
- PBS with 0.1 mg/mL BSA and 0.05 % sodium azide
-
储存液
- Sodium azide
-
注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
储存条件
- -20 °C
-
储存方法
- Aliquot and Store at -20C. Avoid freez-thaw cycles.
-
-
- Melanoma
-
背景
- By immunohistochemistry, this melanoma gp100 antibody specifically recognizes a protein in melanocytes and melanomas. It reacts with junctional and blue nevus cells and variably with fetal and neonatal melanocytes. Intradermal nevi, normal adult melanocytes, and non-melanocytic cells are negative. It does not stain tumor cells of epithelial, lymphoid, glial, or mesenchymal origin. The melanoma antibody labels formalin/paraffin melanomas and other tumors showing melanocytic differentiation.
-
基因ID
- 6490
-
UniProt
- P40967
抗原
-
