Melanoma 抗体
Quick Overview for Melanoma 抗体 (ABIN6252230)
抗原
适用
宿主
克隆类型
标记
应用范围
克隆位点
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特异性
- Does not react with rat
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纯化方法
- Protein G affinity chromatography
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免疫原
- Membranes from a human melanoma metastasis were used as the immunogen for this Melanoma antibody (gp100).
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亚型
- IgG2b kappa
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anti-Melanoma antibody
适用: 人, 马 IHC (p), IF, FACS 宿主: 小鼠 Monoclonal NKI-beteb unconjugated
anti-Melanoma antibody适用: 人, 犬, 小鼠 IHC (p), IF 宿主: 小鼠 Monoclonal PNL2 unconjugated
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应用备注
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Flow Cytometry: 0.5-1 μg/million cells
IF: 1-2 μg/mL
IHC (FFPE): 1-2 μg/mL for 30 minutes at RT (1)
Differences in protocols and secondaries may require the gp100 antibody to be titered up or down for optimal performance.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.2 mg/mL
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缓冲液
- PBS with 0.1 mg/mL BSA and 0.05 % sodium azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- Aliquot and Store at -20C. Avoid freez-thaw cycles.
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- Melanoma
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背景
- By immunohistochemistry, this melanoma gp100 antibody specifically recognizes a protein in melanocytes and melanomas. It reacts with junctional and blue nevus cells and variably with fetal and neonatal melanocytes. Intradermal nevi, normal adult melanocytes, and non-melanocytic cells are negative. It does not stain tumor cells of epithelial, lymphoid, glial, or mesenchymal origin. The melanoma antibody labels formalin/paraffin melanomas and other tumors showing melanocytic differentiation.
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基因ID
- 6490
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UniProt
- P40967
抗原
-
