Nucleolin 抗体
Quick Overview for Nucleolin 抗体 (ABIN6252098)
抗原
See all Nucleolin (NCL) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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纯化方法
- Protein G affinity chromatography
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免疫原
- Recombinant human protein was used as the immunogen for the Nucleolin antibody.
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亚型
- IgG1
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anti-Nucleolin (NCL) (AA 353-568) antibody
NCL 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Nucleolin (NCL) antibodyNCL 适用: 人 WB, IHC, IF, FACS, ISt, StM 宿主: 小鼠 Monoclonal NCL-902 unconjugated
anti-Nucleolin (NCL) (AA 388-569) antibodyNCL 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Nucleolin (NCL) antibodyNCL 适用: 人 WB, IHC, IF, FACS, ISt 宿主: 小鼠 Monoclonal NCL-902 unconjugated
anti-Nucleolin (NCL) antibodyNCL 适用: 人 WB, IHC, IF, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Nucleolin (NCL) antibodyNCL 适用: 人, 小鼠, 大鼠, Cow, Pig, 兔, 豚鼠, 小鸡, Cat, Gerbil WB, IF, FACS, IHC (p) 宿主: 小鼠 Monoclonal NCL-902 unconjugated
anti-Nucleolin (NCL) antibodyNCL 适用: 人, 小鼠, 大鼠, Cow, Pig, 兔, 豚鼠, 小鸡, Cat, Gerbil WB, IF, FACS, IHC (p) 宿主: 小鼠 Monoclonal NCL-902 unconjugated
anti-Nucleolin (NCL) antibodyNCL 适用: 人 WB, IF, FACS, IHC (p) 宿主: 小鼠 Monoclonal 364-5-NCL-902 unconjugated
anti-Nucleolin (NCL) (C-Term) antibodyNCL 适用: 人, 小鼠, 大鼠, Cow, 兔, 犬, 豚鼠, 马 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Nucleolin (NCL) (N-Term) antibodyNCL 适用: 人, 小鼠, 大鼠, Cow, Pig, 兔, 犬, 马, Saccharomyces cerevisiae, 斑马鱼 WB, IHC 宿主: 兔 Polyclonal unconjugated
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应用备注
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IF: 1-2 μg/mL
Flow Cytometry: 0.5-1 μg/million cells in 0.1ml
Western blot: 1-2 μg/mL
Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT (1)
Prediluted format: incubate for 30 min at RT (2)
Titering of the Nucleolin antibody may be required for optimal performance.1. FFPE testing requires sections to be boiled in pH 6 10 mM citrate buffer for 10-20 minutes, followed by cooling at RT for 20 minutes, prior to staining.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min. -
限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.2 mg/mL
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缓冲液
- PBS with 0.1 mg/mL BSA and 0.05 % sodium azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- Aliquot and Store at -20C. Avoid freez-thaw cycles.
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- Nucleolin (NCL)
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别名
- Nucleolin
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背景
- Nucleolin (NCL) is the major nucleolar phosphoprotein of growing eukaryotic cells. It is located mainly in dense fibrillar regions of the nucleolus. NCL is found associated with intranucleolar chromatin and pre-ribosomal particles. It induces chromatin decondensation by binding to histone H1. It is thought to play a role in pre-rRNA transcription and ribosome assembly.
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基因ID
- 4691
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UniProt
- P19338
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途径
- Ribonucleoprotein Complex Subunit Organization
抗原
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