dsDNA 抗体
This 小鼠 单克隆 antibody specifically detects dsDNA in FACS, IF, IHC (p) 和 ICC. It exhibits reactivity toward 人.
产品编号 ABIN6252090
发货至:
中国
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Contact Info
Our Local Distributor
中国
北京 101111
北京 101111
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Beijing Economic Technological Development Area
Room 801-803
Quick Overview for dsDNA 抗体 (ABIN6252090)
抗原
dsDNA
(Double-Stranded DNA (dsDNA))
适用
人
宿主
小鼠
克隆类型
单克隆
标记
This dsDNA antibody is un-conjugated
应用范围
Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)
克隆位点
SPM603
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纯化方法
- Protein G affinity chromatography
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免疫原
- Nuclei of Burkitt's cells were used as the immunogen for the Double Stranded DNA antibody.
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亚型
- IgG3 kappa
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anti-Double-Stranded DNA (dsDNA) antibody
dsDNA 适用: 人 FACS, IHC 宿主: 小鼠 Monoclonal 121-3 unconjugated
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应用备注
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Flow Cytometry: 0.5-1 μg/million cells in 0.1ml
Immunofluorescence: 1-2 μg/mL
Immunocytochemistry (Acetone-fixed): 0.5-1 μg/mL for 30 min at RT
Immunohistochemistry (FFPE): 1-2 μg/mL for 30 min at RT (1)
Optimal dilution of the Double Stranded DNA antibody should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min. -
限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.2 mg/mL
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缓冲液
- PBS with 0.1 mg/mL BSA and 0.05 % sodium azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- Aliquot and Store at -20C. Avoid freez-thaw cycles.
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- dsDNA (Double-Stranded DNA (dsDNA))
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别名
- Double Stranded DNA
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物质类
- Nucleotide
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背景
- Marker antibodies are useful in identification of organelles in cells, tissues, and biochemical preparations. This mAb recognizes the double stranded DNA in human cells. It can be used to stain the nuclei in cell or tissue preparations and can be used as a nuclear marker in human cells. Double stranded (ds) DNA markers are useful tools in biology research and aid in the study of DNA behavior and characteristics.
抗原
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