dsDNA 抗体
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- 抗原 See all dsDNA products
- dsDNA (Double-Stranded DNA (dsDNA))
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适用
- 人
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This dsDNA antibody is un-conjugated
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应用范围
- Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (IHC), Staining Methods (StM)
- 特异性
- This monoclonal antibody is part of a new panel of reagents, which recognizes subcellular organelles or compartments of human cells. These markers may be useful in identification of these organelles in cells, tissues, and biochemical preparations. This MAb recognizes the double stranded DNA in human cells. It can be used to stain the nuclei in cell or tissue preparations and can be used as a nuclear marker in human cells. This MAb produces a homogeneous staining pattern in the nucleus of normal and malignant cells.Deoxyribonucleic acid (DNA) is a long polymer of nucleotides that is held together by a backbone made of sugars and phosphate groups. It holds the genetic instructions for the development and function of living things. DNA is crucial for living organisms, and all known cellular life and some viruses contain DNA. In eukaryotes, DNA exists in the cell nucleus, while in prokaryotes, DNA is located in the cytoplasm. In living organisms, DNA does not usually exist as a single molecule, but instead as a tightly associated pair of molecules in the shape of a right-handed double helix. Hydrogen bonds as well as forces generated by the hydrophobic effect and pi stacking hold the two DNA strands together. During replication and transcription, portions of the helix unwind and become single stranded. Protective proteins surround these single-stranded DNA. Double stranded (ds) DNA markers are useful tools in biology research and aid in the study of DNA behavior and characteristics.
- 免疫原
- Nuclei of Burkitt's cells
- 克隆位点
- 121-3
- 亚型
- IgG3 kappa
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anti-Double-Stranded DNA (dsDNA) antibody
dsDNA 适用: 人 IF, FACS, IHC, StM 宿主: 小鼠 Monoclonal 121-3 unconjugated
anti-Double-Stranded DNA (dsDNA) antibodydsDNA 适用: 人 IF, FACS, ICC, IHC (p) 宿主: 小鼠 Monoclonal DSD-958 unconjugated
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- 应用备注
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Positive Control: Raji, Jurkat or HeLa cells. Tonsil or Colon.
Known Application: Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT) (Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.
- 限制
- 仅限研究用
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- 缓冲液
- 10 mM PBS without BSA and without Azide.
- 储存液
- Azide free
- 储存条件
- 4 °C,-80 °C
- 储存方法
- Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months.
- 有效期
- 24 months
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- 抗原
- dsDNA (Double-Stranded DNA (dsDNA))
- 别名
- Double Stranded DNA (dsDNA) (Nuclear Marker) (dsDNA 产品)
- 物质类
- Chemical
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