hCG Holo 抗体
The 小鼠 单克隆 anti-hCG Holo antibody is suitable to detect hCG Holo in samples from 人. It has been validated for IHC (p) 和 WB.
产品编号 ABIN6252022
发货至:
中国
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Our Local Distributor
中国
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北京 101111
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Quick Overview for hCG Holo 抗体 (ABIN6252022)
抗原
hCG Holo
适用
人
宿主
小鼠
克隆类型
单克隆
标记
非结合性
应用范围
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
克隆位点
HCGab-52
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纯化方法
- Protein G affinity chromatography
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免疫原
- Purified hCG protein was used as the immunogen for the hCG Holo antibody.
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亚型
- IgG1 kappa
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anti-hCG Holo antibody
适用: 人 IHC (p) 宿主: 小鼠 Monoclonal HCGab-52 unconjugated
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应用备注
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Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT (1)
Prediluted format: incubate for 30 min at RT (2)
Optimal dilution of the hCG Holo antibody should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min. -
限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.2 mg/mL
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缓冲液
- PBS with 0.1 mg/mL BSA and 0.05 % sodium azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- Aliquot and Store at -20C. Avoid freez-thaw cycles.
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- hCG Holo
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背景
- This mAb reacts only with the intact-HCG and not with either free alpha- or free beta-chain of HCG. HCG is a glycoprotein and is composed of two non-identical, non-covalently linked polypeptide chains designated as the alpha and beta subunits. The alpha subunit is identical to that of thyroid stimulating hormone (TSH), follicle stimulating hormone (FSH), and luteinizing hormone (LH). HCG is secreted in large quantities by normal trophoblasts. It is present only in trace amounts in non-pregnant urine and sera but rises sharply during pregnancy. HCG mAb detects cells and tumors of trophoblastic origin such as choriocarcinoma. Large cell carcinoma and adenocarcinoma of the lung demonstrate anti-hCG positivity in 90 % and 60 % of cases respectively. 20 % of lung squamous cell carcinomas are positive. HCG expression by non-trophoblastic tumors may indicate aggressive behavior.
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基因ID
- 1081
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UniProt
- P01215, P01233
抗原
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