Epithelial Marker Antigen 抗体
This 小鼠 单克隆 antibody specifically detects Epithelial Marker Antigen in FACS, IF 和 IHC. It exhibits reactivity toward 人.
产品编号 ABIN6252021
发货至:
中国
Contact our Customer Service for availability and price in your country.
Contact Info
Our Local Distributor
中国
北京 101111
北京 101111
No. 88 KeChuang 6th Street
Beijing Economic Technological Development Area
Room 801-803
Quick Overview for Epithelial Marker Antigen 抗体 (ABIN6252021)
抗原
Epithelial Marker Antigen
适用
人
宿主
小鼠
克隆类型
单克隆
标记
非结合性
应用范围
Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (IHC)
克隆位点
MUC1-967
-
-
纯化方法
- Protein G affinity chromatography
-
免疫原
- Human milk fat globule membranes were used as the immunogen for this Epithelial Marker Antigen antibody.
-
亚型
- IgG1 kappa
-
-
anti-Epithelial Marker Antigen antibody
适用: 人 FACS, IF, IHC (p) 宿主: 小鼠 Monoclonal MUC1-967 unconjugated
-
-
应用备注
-
Flow Cytometry: 0.5-1 μg/million cells
IF: 1-2 μg/mL
IHC (FFPE): 0.5-1 μg/mL for 30 minutes at RT (1)
Prediluted format : incubate for 30 min at RT (2)
The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the Epithelial Marker Antigen antibody to be titered up or down for optimal performance.1. FFPE staining REQUIRES boiling sections in 10 mM citrate buffer, pH 6, for 10-20 min followed by cooling at RT for 10-20 min.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min. -
限制
- 仅限研究用
-
-
-
状态
- Liquid
-
浓度
- 0.2 mg/mL
-
缓冲液
- PBS with 0.1 mg/mL BSA and 0.05 % sodium azide
-
储存液
- Sodium azide
-
注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
储存条件
- -20 °C
-
储存方法
- Aliquot and Store at -20C. Avoid freez-thaw cycles.
-
-
- Epithelial Marker Antigen
-
背景
- This Epithelial Membrane Antigen / EMA antibody, also called MUC1 and Mucin-1, recognizes the full-length protein in a glycosylation-independent manner and can bind to the fully glycosylated protein. The dominant epitope of this mAb is APDTR in the VNTR region. It reacts with the core peptide of the EMA protein, which is a member of a family of mucin glycoproteins that are characterized by high carbohydrate content, O-linked oligosaccharides, high molecular weight (>200 kDa) and an amino acid composition rich in serine, threonine, proline and glycine. The core protein contains a domain of 20 amino-acid tandem repeats that functions as multiple epitopes for the mAb. Incomplete glycosylation of some tumor-associated mucins may lead to variable unmasking of the multiple peptide epitopes leading to the observed differences in staining intensity between normal and malignant tissues. This EMA antibody reacts with both normal and malignant epithelia of various tissues including breast and colon.
-
基因ID
- 4582
-
UniProt
- P15941
抗原
-
You are here:
