Epithelial Marker Antigen 抗体
The 小鼠 单克隆 anti-Epithelial Marker Antigen antibody is suitable to detect Epithelial Marker Antigen in samples from 人. It has been validated for FACS, IF 和 IHC (p).
产品编号 ABIN3026793
发货至:
中国
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Contact Info
Our Local Distributor
中国
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北京 101111
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Quick Overview for Epithelial Marker Antigen 抗体 (ABIN3026793)
抗原
Epithelial Marker Antigen
适用
人
宿主
小鼠
克隆类型
单克隆
标记
非结合性
应用范围
Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
克隆位点
MUC1-967
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纯化方法
- Protein G affinity chromatography
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纯度
- Protein G affinity chromatography
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免疫原
- Human milk fat globule membranes were used as the immunogen for this Epithelial Marker Antigen antibody.
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亚型
- IgG1 kappa
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应用备注
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The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the Epithelial Marker Antigen antibody to be titered up or down for optimal performance.
1. FFPE staining REQUIRES boiling sections in 10 mM citrate buffer, pH 6, for 10-20 min followed by cooling at RT for 10-20 min.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. FACS: 0.5-1 μg/million cells,IF: 1-2 μg/mL,IHC (FFPE): 0.5-1 μg/mL for 30 minutes at RT (1),Prediluted format : incubate for 30 min at RT (2) -
限制
- 仅限研究用
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浓度
- 1 mg/mL
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缓冲液
- 1 mg/mL in 1X PBS, BSA free, sodium azide free
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储存液
- Azide free
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储存条件
- 4 °C,-20 °C
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储存方法
- Store the Epithelial marker antigen antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
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- Epithelial Marker Antigen
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背景
- This Epithelial Membrane Antigen / EMA antibody, also called MUC1 and Mucin-1, recognizes the full-length protein in a glycosylation-independent manner and can bind to the fully glycosylated protein. The dominant epitope of this mAb is APDTR in the VNTR region. It reacts with the core peptide of the EMA protein, which is a member of a family of mucin glycoproteins that are characterized by high carbohydrate content, O-linked oligosaccharides, high molecular weight (>200 kDa) and an amino acid composition rich in serine, threonine, proline and glycine. The core protein contains a domain of 20 amino-acid tandem repeats that functions as multiple epitopes for the mAb. Incomplete glycosylation of some tumor-associated mucins may lead to variable unmasking of the multiple peptide epitopes leading to the observed differences in staining intensity between normal and malignant tissues. This EMA antibody reacts with both normal and malignant epithelia of various tissues including breast and colon.
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基因ID
- 4582
抗原
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