Golgi 抗体
This anti- antibody is a 小鼠 单克隆 antibody detecting in IHC (p), FACS 和 IF. Suitable for 人.
Quick Overview for Golgi 抗体 (ABIN6251933)
抗原
Golgi
适用
人
宿主
小鼠
克隆类型
单克隆
标记
非结合性
应用范围
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Flow Cytometry (FACS), Immunofluorescence (IF)
克隆位点
CRFG1
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纯化方法
- Protein G affinity chromatography
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免疫原
- The Golgi fraction from human liver cells was used as the immunogen for the Golgi antibody. Not suitable for mouse and rat sample testing.
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亚型
- IgG1 kappa
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anti-Golgi antibody
适用: 人 IHC (p), FACS, IF, ICC, WB 宿主: 小鼠 Monoclonal AE-6 unconjugated
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应用备注
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Flow Cytometry: 0.5-1 μg/million cells in 0.1ml
Immunofluorescence: 1-2 μg/mL
Immunohistochemistry (FFPE): 1-2 μg/mL for 30 min at RT (1)
Prediluted format : incubate for 30 min at RT (2)
Titering of the Golgi antibody may be required for optimal performance.1. FFPE testing requires sections to be boiled in pH 6 10 mM citrate buffer for 10-20 minutes, followed by cooling at RT for 20 minutes, prior to staining.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min. -
限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.2 mg/mL
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缓冲液
- PBS with 0.1 mg/mL BSA and 0.05 % sodium azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- Aliquot and Store at -20C. Avoid freez-thaw cycles.
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- Golgi
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背景
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Part of the cellular endomembrane system, the Golgi apparatus packages proteins into membrane-bound vesicles inside the cell before the vesicles are sent to their destination. The Golgi apparatus resides at the intersection of the secretory, lysosomal, and endocytic pathways. It is of particular importance in processing proteins for secretion, containing a set of glycosylation enzymes that attach various sugar monomers to proteins as the proteins move through the apparatus. [Wiki]
This antibody is useful in staining the Golgi apparatus in cell or tissue preparations and subcellular fractions. -
基因ID
- 2734
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UniProt
- Q92896
抗原
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